@article{1a8eab9c6d9f41a4a282775eb8b9b8a1,
title = "INCREASED OESTRADIOL IN DIAZEPAM RELATED GYNAECOMASTIA",
author = "Donald Bergman and Walter Futterweit and Robert Segal and David Sirota",
note = "Funding Information: children with CF. No difference was found between the CF fluids and the controls. All three are within the normal range. After isoelectric focusing of the CF fluids and staining for protease activity with N-benzoylarginine ethyl ester (BAEE) two of the fluids (B and C) were normal; testing of the fluid from twin A was inconclusive because of the very small amount of fluid available. We have been able to distinguish activated plasma from controls and patients by this method. However, only around 50\% of our CF patients investigated so far exhibit the abnormal isoelectric focusing pattern with a missing protease band. Plasma from three CF children was therefore subjected to isoelectric focusing and BAEE staining. Two of the three patients (B and C) did show normal banding with five protease activity bands, whereas twin A2 had only four. The MUGB reactivity values would have misdiagnosed all three cases and isoelectric focusing would have missed at least two, predicting normal outcome if prenatal diagnoses had been attempted. We conclude that prenatal diagnosis of CF should only be offered to women at risk when index patient has been identified by the above mentioned methods. One of us (M. S.) thanks Dr H. L. Nadler and his colleagues, who taught her the methods during a stay at his laboratory. This work was supported by grants from the Danish Medical Research Council, the Queen Louise Foundation, and the National Danish Association against Cystic Fibrosis.",
year = "1981",
month = nov,
day = "28",
doi = "10.1016/S0140-6736(81)91456-2",
language = "English",
volume = "318",
pages = "1225--1226",
journal = "The Lancet",
issn = "0140-6736",
publisher = "Elsevier B.V.",
number = "8257",
}