Increased nitration and diminished activity of copper/zinc superoxide dismutase in placentas from diabetic rats

Verónica White; Evangelina Capobianco; Romina Higa; Nora Martínez; María Sosa; María C. Pustovrh; Alicia Jawerbaum

doi:10.3109/10715762.2010.512039

Increased nitration and diminished activity of copper/zinc superoxide dismutase in placentas from diabetic rats

Verónica White
, Evangelina Capobianco
, Romina Higa
, Nora Martínez
, María Sosa
, María C. Pustovrh
, Alicia Jawerbaum

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Nitration-induced protein damage in the placenta leads to impaired blood flow and deficient fetoplacental exchange in diabetic pregnancies. This work studied the effect of nitric oxide and peroxynitrite on Cu/Zn SOD activity in rat placentas and evaluated whether Cu/Zn SOD is nitrated in the placenta from diabetic rats at mid-gestation. Protein nitration was evaluated by EIA, Cu/Zn SOD activity by inhibition of the epinephrine auto-oxidation, Cu/Zn SOD expression by western blot and specific nitration by immunoprecipitation. This study found higher levels of protein nitration (p < 0.001), diminished Cu/Zn SOD activity and enhanced protein expression (p < 0.01) in placentas from diabetic rats. Placental Cu/Zn SOD activity was inhibited by peroxynitrite (p < 0.01). Besides, nitration of Cu/Zn SOD was elevated in placentas from diabetic rats (p < 0.01). These results show that rat Cu/Zn SOD can be nitrated, a modification that could lead to the depressed activity of this enzyme found in placentas from diabetic rats.

Original language	English
Pages (from-to)	1407-1415
Number of pages	9
Journal	Free Radical Research
Volume	44
Issue number	12
DOIs	https://doi.org/10.3109/10715762.2010.512039
State	Published - Dec 2010
Externally published	Yes

Keywords

Cu/Zn SOD
Placenta
diabetes
peroxynitrite
rat

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10.3109/10715762.2010.512039

Cite this

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title = "Increased nitration and diminished activity of copper/zinc superoxide dismutase in placentas from diabetic rats",

abstract = "Nitration-induced protein damage in the placenta leads to impaired blood flow and deficient fetoplacental exchange in diabetic pregnancies. This work studied the effect of nitric oxide and peroxynitrite on Cu/Zn SOD activity in rat placentas and evaluated whether Cu/Zn SOD is nitrated in the placenta from diabetic rats at mid-gestation. Protein nitration was evaluated by EIA, Cu/Zn SOD activity by inhibition of the epinephrine auto-oxidation, Cu/Zn SOD expression by western blot and specific nitration by immunoprecipitation. This study found higher levels of protein nitration (p < 0.001), diminished Cu/Zn SOD activity and enhanced protein expression (p < 0.01) in placentas from diabetic rats. Placental Cu/Zn SOD activity was inhibited by peroxynitrite (p < 0.01). Besides, nitration of Cu/Zn SOD was elevated in placentas from diabetic rats (p < 0.01). These results show that rat Cu/Zn SOD can be nitrated, a modification that could lead to the depressed activity of this enzyme found in placentas from diabetic rats.",

keywords = "Cu/Zn SOD, Placenta, diabetes, peroxynitrite, rat",

author = "Ver{\'o}nica White and Evangelina Capobianco and Romina Higa and Nora Mart{\'i}nez and Mar{\'i}a Sosa and Pustovrh, \{Mar{\'i}a C.\} and Alicia Jawerbaum",

note = "Funding Information: Declaration of interest: This work was supported by grants from Agencia de Promoci {\'o} n Cient {\'i} fica y Tecnol gica de Argentina (PICT 07-01958, V.W and PICT 05-32268, A.J.) and from Ministerio de Salud Argentina (Ram {\'o} n Carrillo-Arturo O {\~n} ativia).",

year = "2010",

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doi = "10.3109/10715762.2010.512039",

language = "English",

volume = "44",

pages = "1407--1415",

journal = "Free Radical Research",

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TY - JOUR

T1 - Increased nitration and diminished activity of copper/zinc superoxide dismutase in placentas from diabetic rats

AU - White, Verónica

AU - Capobianco, Evangelina

AU - Higa, Romina

AU - Martínez, Nora

AU - Sosa, María

AU - Pustovrh, María C.

AU - Jawerbaum, Alicia

N1 - Funding Information: Declaration of interest: This work was supported by grants from Agencia de Promoci ó n Cient í fica y Tecnol gica de Argentina (PICT 07-01958, V.W and PICT 05-32268, A.J.) and from Ministerio de Salud Argentina (Ram ó n Carrillo-Arturo O ñ ativia).

PY - 2010/12

Y1 - 2010/12

N2 - Nitration-induced protein damage in the placenta leads to impaired blood flow and deficient fetoplacental exchange in diabetic pregnancies. This work studied the effect of nitric oxide and peroxynitrite on Cu/Zn SOD activity in rat placentas and evaluated whether Cu/Zn SOD is nitrated in the placenta from diabetic rats at mid-gestation. Protein nitration was evaluated by EIA, Cu/Zn SOD activity by inhibition of the epinephrine auto-oxidation, Cu/Zn SOD expression by western blot and specific nitration by immunoprecipitation. This study found higher levels of protein nitration (p < 0.001), diminished Cu/Zn SOD activity and enhanced protein expression (p < 0.01) in placentas from diabetic rats. Placental Cu/Zn SOD activity was inhibited by peroxynitrite (p < 0.01). Besides, nitration of Cu/Zn SOD was elevated in placentas from diabetic rats (p < 0.01). These results show that rat Cu/Zn SOD can be nitrated, a modification that could lead to the depressed activity of this enzyme found in placentas from diabetic rats.

AB - Nitration-induced protein damage in the placenta leads to impaired blood flow and deficient fetoplacental exchange in diabetic pregnancies. This work studied the effect of nitric oxide and peroxynitrite on Cu/Zn SOD activity in rat placentas and evaluated whether Cu/Zn SOD is nitrated in the placenta from diabetic rats at mid-gestation. Protein nitration was evaluated by EIA, Cu/Zn SOD activity by inhibition of the epinephrine auto-oxidation, Cu/Zn SOD expression by western blot and specific nitration by immunoprecipitation. This study found higher levels of protein nitration (p < 0.001), diminished Cu/Zn SOD activity and enhanced protein expression (p < 0.01) in placentas from diabetic rats. Placental Cu/Zn SOD activity was inhibited by peroxynitrite (p < 0.01). Besides, nitration of Cu/Zn SOD was elevated in placentas from diabetic rats (p < 0.01). These results show that rat Cu/Zn SOD can be nitrated, a modification that could lead to the depressed activity of this enzyme found in placentas from diabetic rats.

KW - Cu/Zn SOD

KW - Placenta

KW - diabetes

KW - peroxynitrite

KW - rat

UR - https://www.scopus.com/pages/publications/78149268415

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DO - 10.3109/10715762.2010.512039

M3 - Article

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EP - 1415

JO - Free Radical Research

JF - Free Radical Research

IS - 12

ER -

Increased nitration and diminished activity of copper/zinc superoxide dismutase in placentas from diabetic rats

Abstract

Keywords

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