In vivo micro magnetic resonance imaging signal changes in scrapie infected mice

Marcin Sadowski; Cheuk Ying Tang; Juan Gilberto Aguinaldo; Richard Carp; Harry C. Meeker; Thomas Wisniewski

doi:10.1016/S0304-3940(03)00319-7

In vivo micro magnetic resonance imaging signal changes in scrapie infected mice

Marcin Sadowski
, Cheuk Ying Tang
, Juan Gilberto Aguinaldo
, Richard Carp
, Harry C. Meeker
, Thomas Wisniewski

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Signal abnormalities on magnetic resonance imaging (MRI) T2-weighted images (T2WI) have been described in patients with Creutzfeldt-Jakob disease; however, the pathology underlying these findings remains to be fully described. We investigated the time-course of signal alterations in a murine model of prion disease using in vivo 9.4 Tesla micro magnetic resonance imaging (μMRI). The topography of μMRI signal changes was correlated with the accumulation of proteinase resistant PrP^Sc in corresponding brain sections. Increased signal intensity on T2WI was observed in the septum and in the hippocampus of presymptomatic mice 120 days post infection (dpi). Mildly symptomatic animals (150 dpi) and animals with apparent neurological deficit (180 dpi) had a greater increase of signal intensity on T2WI in the septum and the hippocampus; in addition, abnormalities in the cortex and in the thalamus were found. Neuropathological evaluation demonstrated accumulation of PrP^Sc and astrogliosis but only minimal or no spongiform changes in structures where abnormal signal was detected. These observations suggest that early pathological changes related to the accumulation of PrP^Sc may be detectable in presymptomatic subjects using MRI systems with higher magnetic field strength.

Original language	English
Pages (from-to)	1-4
Number of pages	4
Journal	Neuroscience Letters
Volume	345
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3940(03)00319-7
State	Published - 10 Jul 2003

Keywords

Conformational disorder
Histoblot
Magnetic resonance imaging
Mouse
Prion
Proteinase K
T2-weighted image

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10.1016/S0304-3940(03)00319-7

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@article{176525b96bd147af97df0856c7f13788,

title = "In vivo micro magnetic resonance imaging signal changes in scrapie infected mice",

abstract = "Signal abnormalities on magnetic resonance imaging (MRI) T2-weighted images (T2WI) have been described in patients with Creutzfeldt-Jakob disease; however, the pathology underlying these findings remains to be fully described. We investigated the time-course of signal alterations in a murine model of prion disease using in vivo 9.4 Tesla micro magnetic resonance imaging (μMRI). The topography of μMRI signal changes was correlated with the accumulation of proteinase resistant PrPSc in corresponding brain sections. Increased signal intensity on T2WI was observed in the septum and in the hippocampus of presymptomatic mice 120 days post infection (dpi). Mildly symptomatic animals (150 dpi) and animals with apparent neurological deficit (180 dpi) had a greater increase of signal intensity on T2WI in the septum and the hippocampus; in addition, abnormalities in the cortex and in the thalamus were found. Neuropathological evaluation demonstrated accumulation of PrPSc and astrogliosis but only minimal or no spongiform changes in structures where abnormal signal was detected. These observations suggest that early pathological changes related to the accumulation of PrPSc may be detectable in presymptomatic subjects using MRI systems with higher magnetic field strength.",

keywords = "Conformational disorder, Histoblot, Magnetic resonance imaging, Mouse, Prion, Proteinase K, T2-weighted image",

author = "Marcin Sadowski and Tang, \{Cheuk Ying\} and Aguinaldo, \{Juan Gilberto\} and Richard Carp and Meeker, \{Harry C.\} and Thomas Wisniewski",

note = "Funding Information: The authors wish to thank Dr Man-Sun Sy for providing monoclonal antibodies. This work was supported by the Alzheimer's Disease Association and NIH grants AG20747, AG17617 and AG20245.",

year = "2003",

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T1 - In vivo micro magnetic resonance imaging signal changes in scrapie infected mice

AU - Sadowski, Marcin

AU - Tang, Cheuk Ying

AU - Aguinaldo, Juan Gilberto

AU - Carp, Richard

AU - Meeker, Harry C.

AU - Wisniewski, Thomas

N1 - Funding Information: The authors wish to thank Dr Man-Sun Sy for providing monoclonal antibodies. This work was supported by the Alzheimer's Disease Association and NIH grants AG20747, AG17617 and AG20245.

PY - 2003/7/10

Y1 - 2003/7/10

N2 - Signal abnormalities on magnetic resonance imaging (MRI) T2-weighted images (T2WI) have been described in patients with Creutzfeldt-Jakob disease; however, the pathology underlying these findings remains to be fully described. We investigated the time-course of signal alterations in a murine model of prion disease using in vivo 9.4 Tesla micro magnetic resonance imaging (μMRI). The topography of μMRI signal changes was correlated with the accumulation of proteinase resistant PrPSc in corresponding brain sections. Increased signal intensity on T2WI was observed in the septum and in the hippocampus of presymptomatic mice 120 days post infection (dpi). Mildly symptomatic animals (150 dpi) and animals with apparent neurological deficit (180 dpi) had a greater increase of signal intensity on T2WI in the septum and the hippocampus; in addition, abnormalities in the cortex and in the thalamus were found. Neuropathological evaluation demonstrated accumulation of PrPSc and astrogliosis but only minimal or no spongiform changes in structures where abnormal signal was detected. These observations suggest that early pathological changes related to the accumulation of PrPSc may be detectable in presymptomatic subjects using MRI systems with higher magnetic field strength.

AB - Signal abnormalities on magnetic resonance imaging (MRI) T2-weighted images (T2WI) have been described in patients with Creutzfeldt-Jakob disease; however, the pathology underlying these findings remains to be fully described. We investigated the time-course of signal alterations in a murine model of prion disease using in vivo 9.4 Tesla micro magnetic resonance imaging (μMRI). The topography of μMRI signal changes was correlated with the accumulation of proteinase resistant PrPSc in corresponding brain sections. Increased signal intensity on T2WI was observed in the septum and in the hippocampus of presymptomatic mice 120 days post infection (dpi). Mildly symptomatic animals (150 dpi) and animals with apparent neurological deficit (180 dpi) had a greater increase of signal intensity on T2WI in the septum and the hippocampus; in addition, abnormalities in the cortex and in the thalamus were found. Neuropathological evaluation demonstrated accumulation of PrPSc and astrogliosis but only minimal or no spongiform changes in structures where abnormal signal was detected. These observations suggest that early pathological changes related to the accumulation of PrPSc may be detectable in presymptomatic subjects using MRI systems with higher magnetic field strength.

KW - Conformational disorder

KW - Histoblot

KW - Magnetic resonance imaging

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KW - Prion

KW - Proteinase K

KW - T2-weighted image

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In vivo micro magnetic resonance imaging signal changes in scrapie infected mice

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Keywords

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