TY - JOUR
T1 - In vivo imaging of cerebral glucose metabolism informs on subacute to chronic post-stroke tissue status – A pilot study combining PET and deuterium metabolic imaging
AU - Meerwaldt, Anu E.
AU - Straathof, Milou
AU - Oosterveld, Wija
AU - van Heijningen, Caroline L.
AU - van Leent, Mandy M.T.
AU - Toner, Yohana C.
AU - Munitz, Jazz
AU - Teunissen, Abraham J.P.
AU - Daemen, Charlotte C.
AU - van der Toorn, Annette
AU - van Vliet, Gerard
AU - van Tilborg, Geralda A.F.
AU - De Feyter, Henk M.
AU - de Graaf, Robin A.
AU - Hol, Elly M.
AU - Mulder, Willem J.M.
AU - Dijkhuizen, Rick M.
N1 - Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was funded in part by grants from the Netherlands Organisation for Scientific Research (NWO, Graduate Program NCU: 022.006.001). Acknowledgements
Publisher Copyright:
© The Author(s) 2023.
PY - 2023/5
Y1 - 2023/5
N2 - Recanalization therapy after acute ischemic stroke enables restoration of cerebral perfusion. However, a significant subset of patients has poor outcome, which may be caused by disruption of cerebral energy metabolism. To assess changes in glucose metabolism subacutely and chronically after recanalization, we applied two complementary imaging techniques, fluorodeoxyglucose (FDG) positron emission tomography (PET) and deuterium (2H) metabolic imaging (DMI), after 60-minute transient middle cerebral artery occlusion (tMCAO) in C57BL/6 mice. Glucose uptake, measured with FDG PET, was reduced at 48 hours after tMCAO and returned to baseline value after 11 days. DMI revealed effective glucose supply as well as elevated lactate production and reduced glutamate/glutamine synthesis in the lesion area at 48 hours post-tMCAO, of which the extent was dependent on stroke severity. A further decrease in oxidative metabolism was evident after 11 days. Immunohistochemistry revealed significant glial activation in and around the lesion, which may play a role in the observed metabolic profiles. Our findings indicate that imaging (altered) active glucose metabolism in and around reperfused stroke lesions can provide substantial information on (secondary) pathophysiological changes in post-ischemic brain tissue.
AB - Recanalization therapy after acute ischemic stroke enables restoration of cerebral perfusion. However, a significant subset of patients has poor outcome, which may be caused by disruption of cerebral energy metabolism. To assess changes in glucose metabolism subacutely and chronically after recanalization, we applied two complementary imaging techniques, fluorodeoxyglucose (FDG) positron emission tomography (PET) and deuterium (2H) metabolic imaging (DMI), after 60-minute transient middle cerebral artery occlusion (tMCAO) in C57BL/6 mice. Glucose uptake, measured with FDG PET, was reduced at 48 hours after tMCAO and returned to baseline value after 11 days. DMI revealed effective glucose supply as well as elevated lactate production and reduced glutamate/glutamine synthesis in the lesion area at 48 hours post-tMCAO, of which the extent was dependent on stroke severity. A further decrease in oxidative metabolism was evident after 11 days. Immunohistochemistry revealed significant glial activation in and around the lesion, which may play a role in the observed metabolic profiles. Our findings indicate that imaging (altered) active glucose metabolism in and around reperfused stroke lesions can provide substantial information on (secondary) pathophysiological changes in post-ischemic brain tissue.
KW - Cerebral glucose metabolism
KW - deuterium metabolic imaging
KW - ischemic stroke
KW - magnetic resonance imaging/spectroscopy
KW - positron emission tomography
UR - http://www.scopus.com/inward/record.url?scp=85146074399&partnerID=8YFLogxK
U2 - 10.1177/0271678X221148970
DO - 10.1177/0271678X221148970
M3 - Article
C2 - 36606595
AN - SCOPUS:85146074399
SN - 0271-678X
VL - 43
SP - 778
EP - 790
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
IS - 5
ER -