In vivo bioluminescent imaging of influenza a virus infection and characterization of novel cross-protective monoclonal antibodies

Nicholas S. Heaton, Victor H. Leyva-Grado, Gene S. Tan, Dirk Eggink, Rong Hai, Peter Palese

Research output: Contribution to journalArticlepeer-review

124 Scopus citations

Abstract

Influenza A virus is a major human pathogen responsible for seasonal epidemics as well as pandemic outbreaks. Due to the continuing burden on human health, the need for new tools to study influenza virus pathogenesis as well as to evaluate new herapeutics is paramount. We report the development of a stable, eplication-competent luciferase reporter influenza A virus that can be used for in vivo imaging of viral replication. This imaging is noninvasive and allows for the longitudinal monitoring of infection in living animals. We used this tool to characterize novel monoclonal antibodies that bind the conserved stalk domain of the viral hemagglutinin of H1 and H5 subtypes and protect mice from lethal disease. The use of luciferase reporter influenza viruses allows for new mechanistic studies to expand our knowledge of virus-induced disease and provides a new quantitative method to evaluate future antiviral therapies.

Original languageEnglish
Pages (from-to)8272-8281
Number of pages10
JournalJournal of Virology
Volume87
Issue number15
DOIs
StatePublished - 2013

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