In vivo assessment of behavioral recovery and circulatory exchange in the peritoneal parabiosis model

Joseph M. Castellano, Mikael Palner, Shi Bin Li, G. Mark Freeman, Andy Nguyen, Bin Shen, Trisha Stan, Kira I. Mosher, Frederick T. Chin, Luis De Lecea, Jian Luo, Tony Wyss-Coray

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


The sharing of circulation between two animals using a surgical procedure known as parabiosis has created a wealth of information towards our understanding of physiology, most recently in the neuroscience arena. The systemic milieu is a complex reservoir of tissues, immune cells, and circulating molecules that is surprisingly not well understood in terms of its communication across organ systems. While the model has been used to probe complex physiological questions for many years, critical parameters of recovery and exchange kinetics remain incompletely characterized, limiting the ability to design experiments and interpret results for complex questions. Here we provide evidence that mice joined by parabiosis gradually recover much physiology relevant to the study of brain function. Specifically, we describe the timecourse for a variety of recovery parameters, including those for general health and metabolism, motor coordination, activity, and sleep behavior. Finally, we describe the kinetics of chimerism for several lymphocyte populations as well as the uptake of small molecules into the brains of mice following parabiosis. Our characterization provides an important resource to those attempting to understand the complex interplay between the immune system and the brain as well as other organ systems.

Original languageEnglish
Article number29015
JournalScientific Reports
StatePublished - 1 Jul 2016
Externally publishedYes


Dive into the research topics of 'In vivo assessment of behavioral recovery and circulatory exchange in the peritoneal parabiosis model'. Together they form a unique fingerprint.

Cite this