TY - JOUR
T1 - In vitro translation of influenza virus messenger RNAs
AU - Ritchey, M. B.
AU - Palese, P.
N1 - Funding Information:
This research was supported by Grant No. AI-11823o f the NIH. M.B.R. is supported by the NIH Training Grant No. l-TOl-AI-00439.
PY - 1976/7/15
Y1 - 1976/7/15
N2 - A wheat embryo translation system was used to study the translation of influenza virus messenger RNAs. In this in vitro system, the influenza virus proteins, P1 and P2, the nucleoprotein NP, the matrix protein M, and the nonstructural protein NS1 were made but synthesis of the glycosylated proteins, the hemagglutinin, and the neuraminidase was not detected. Products of the in vitro reaction were identified by polyacrylamide-gel electrophoresis and tryptic peptide maps. This confirmed the existence of the nonstructural protein NS1 as a protein separate from the M protein. No evidence was found in the in vitro system for the presence of NS2, a low molecular weight nonstructural protein, which has been described in the literature.
AB - A wheat embryo translation system was used to study the translation of influenza virus messenger RNAs. In this in vitro system, the influenza virus proteins, P1 and P2, the nucleoprotein NP, the matrix protein M, and the nonstructural protein NS1 were made but synthesis of the glycosylated proteins, the hemagglutinin, and the neuraminidase was not detected. Products of the in vitro reaction were identified by polyacrylamide-gel electrophoresis and tryptic peptide maps. This confirmed the existence of the nonstructural protein NS1 as a protein separate from the M protein. No evidence was found in the in vitro system for the presence of NS2, a low molecular weight nonstructural protein, which has been described in the literature.
UR - https://www.scopus.com/pages/publications/0017182428
U2 - 10.1016/0042-6822(76)90170-7
DO - 10.1016/0042-6822(76)90170-7
M3 - Article
C2 - 948868
AN - SCOPUS:0017182428
SN - 0042-6822
VL - 72
SP - 410
EP - 420
JO - Virology
JF - Virology
IS - 2
ER -