In vitro produced monoclonal rheumatoid factor: Purification, radiolabel, and possible applications

Michael Steinitz; Norio Koide; Gad Spira; Sara Tamir; George Klein

doi:10.1016/0008-8749(82)90067-3

In vitro produced monoclonal rheumatoid factor: Purification, radiolabel, and possible applications

Michael Steinitz, Norio Koide, Gad Spira, Sara Tamir, George Klein

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A human monoclonal rheumatoid factor (IgM, λ) produced in vitro by Epstein-Barr virus (EBV) immortalized cell line was purified from cell culture medium. Purification, based on the binding of this antibody to protein A, was carried out by adsorption on protein A-Sepharose and elution with low pH buffer. The eluted preparation (×400 purified) was radiolabeled. The specific binding of the purified rheumatoid factor (RF) to immune complexes was kept intact. This unique human monoclonal antibody is applied as a universal reagent to detect low titers of antibodies directed toward cellular antigens.

Original language	English
Pages (from-to)	205-214
Number of pages	10
Journal	Cellular Immunology
Volume	69
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(82)90067-3
State	Published - 15 May 1982
Externally published	Yes

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title = "In vitro produced monoclonal rheumatoid factor: Purification, radiolabel, and possible applications",

abstract = "A human monoclonal rheumatoid factor (IgM, λ) produced in vitro by Epstein-Barr virus (EBV) immortalized cell line was purified from cell culture medium. Purification, based on the binding of this antibody to protein A, was carried out by adsorption on protein A-Sepharose and elution with low pH buffer. The eluted preparation (×400 purified) was radiolabeled. The specific binding of the purified rheumatoid factor (RF) to immune complexes was kept intact. This unique human monoclonal antibody is applied as a universal reagent to detect low titers of antibodies directed toward cellular antigens.",

author = "Michael Steinitz and Norio Koide and Gad Spira and Sara Tamir and George Klein",

note = "Funding Information: This work was carried out with the support work reported in this paper was undertaken the European Molecular Biology Organization Against Cancer (Yamagiwa-Yoshida Memorial) of a Volkswagen Stiftung Grant AZ: I/36 120-121. The during the tenure of a short time fellowship awarded by to Michael Steinitz and by the International Union to Gad Spira.",

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doi = "10.1016/0008-8749(82)90067-3",

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T1 - In vitro produced monoclonal rheumatoid factor

T2 - Purification, radiolabel, and possible applications

AU - Steinitz, Michael

AU - Koide, Norio

AU - Spira, Gad

AU - Tamir, Sara

AU - Klein, George

N1 - Funding Information: This work was carried out with the support work reported in this paper was undertaken the European Molecular Biology Organization Against Cancer (Yamagiwa-Yoshida Memorial) of a Volkswagen Stiftung Grant AZ: I/36 120-121. The during the tenure of a short time fellowship awarded by to Michael Steinitz and by the International Union to Gad Spira.

PY - 1982/5/15

Y1 - 1982/5/15

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AB - A human monoclonal rheumatoid factor (IgM, λ) produced in vitro by Epstein-Barr virus (EBV) immortalized cell line was purified from cell culture medium. Purification, based on the binding of this antibody to protein A, was carried out by adsorption on protein A-Sepharose and elution with low pH buffer. The eluted preparation (×400 purified) was radiolabeled. The specific binding of the purified rheumatoid factor (RF) to immune complexes was kept intact. This unique human monoclonal antibody is applied as a universal reagent to detect low titers of antibodies directed toward cellular antigens.

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In vitro produced monoclonal rheumatoid factor: Purification, radiolabel, and possible applications

Abstract

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