In vitro precursor-directed synthesis of polyketide analogues with coenzyme a regeneration for the development of antiangiogenic agents

Moon I.I. Kim, Seok Joon Kwon, Jonathan S. Dordick

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Polyketide analogues are produced via in vitro reconstruction of a precursor-directed polyketide biosynthetic pathway. Malonyl-CoA synthetase (MCS) was used in conjunction with chalcone synthase (CHS), thereby allowing efficient use of synthetic starter molecules and malonate as extender. Coenzyme-A was recycled up to 50 times. The use of a simple immobilization procedure resulted in up to a 30-fold higher yield of pyrone CHS products than that obtained with the free enzyme solutions.

Original languageEnglish
Pages (from-to)3806-3809
Number of pages4
JournalOrganic Letters
Volume11
Issue number17
DOIs
StatePublished - 3 Sep 2009
Externally publishedYes

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