TY - JOUR
T1 - In Vitro Models for the Study of Liver Biology and Diseases
T2 - Advances and Limitations
AU - Kaur, Savneet
AU - Kidambi, Srivatsan
AU - Ortega-Ribera, Martí
AU - Thuy, Le Thi Thanh
AU - Nieto, Natalia
AU - Cogger, Victoria C.
AU - Xie, Wei Fen
AU - Tacke, Frank
AU - Gracia-Sancho, Jordi
N1 - Funding Information:
Funding Supported by ASEAN grant CRD/2019/000120 from the Department of Science and Technology, India (S.K.), by National Institutes of Health grants 1R01AA027189-01A1 and P20 GM104320 to the Nebraska Center for the Prevention of Obesity Diseases, grant P20 GM113126 to the Nebraska Center for Integrated Biomolecular Communication, and a Nebraska Research Initiative-systems grant (S.K.). Supported by grants-in-aid for scientific research from the Japan Society for the Promotion of Science JSPS-22K08083 and 22H03061, and a grant for the Research Program on Hepatitis from the Japan Agency for Medical Research and Development (AMED-JP22fk0210107) (L.T.T.T.). Funded by US Public Health Service grant R01 DK111677 from the National Institute of Diabetes and Digestive and Kidney Diseases, grant R01AA025907 from the National Institute on Alcohol Abuse and Alcoholism, and US Veterans Administration grant I01BX005093 from the Biomedical Laboratory Research and Development (N.N.). Supported by the McKnight Philanthropic Bequest and National Health and Medical Research Council (V.C.C.); by National Natural Science Foundation of China grant 82030021 (W.-F.X.); by German Research Foundation grants DFG SFB/TRR 296 and CRC1382 (project-ID 403224013), and the German Ministry of Education and Research (BMBF DEEP-HCC consortium) (F.T.); and by Instituto de Salud Carlos III grant FIS PI20/00220 (co-funded by the European Union), the CIBEREHD, Swiss National Science Funds 320030_189252, the Novartis Foundation for Medical-Biological Research, and the Foundation Suisse Contre le Cancer du Foie (J.G.-S.).
Publisher Copyright:
© 2022 The Authors
PY - 2023/1
Y1 - 2023/1
N2 - In vitro models of liver (patho)physiology, new technologies, and experimental approaches are progressing rapidly. Based on cell lines, induced pluripotent stem cells or primary cells derived from mouse or human liver as well as whole tissue (slices), such in vitro single- and multicellular models, including complex microfluidic organ-on-a-chip systems, provide tools to functionally understand mechanisms of liver health and disease. The International Society of Hepatic Sinusoidal Research (ISHSR) commissioned this working group to review the currently available in vitro liver models and describe the advantages and disadvantages of each in the context of evaluating their use for the study of liver functionality, disease modeling, therapeutic discovery, and clinical applicability.
AB - In vitro models of liver (patho)physiology, new technologies, and experimental approaches are progressing rapidly. Based on cell lines, induced pluripotent stem cells or primary cells derived from mouse or human liver as well as whole tissue (slices), such in vitro single- and multicellular models, including complex microfluidic organ-on-a-chip systems, provide tools to functionally understand mechanisms of liver health and disease. The International Society of Hepatic Sinusoidal Research (ISHSR) commissioned this working group to review the currently available in vitro liver models and describe the advantages and disadvantages of each in the context of evaluating their use for the study of liver functionality, disease modeling, therapeutic discovery, and clinical applicability.
KW - Bioengineering
KW - Cirrhosis
KW - Hepatic Sinusoid
KW - Mechanobiology
KW - NAFLD
KW - NASH
KW - Omics
UR - http://www.scopus.com/inward/record.url?scp=85146450317&partnerID=8YFLogxK
U2 - 10.1016/j.jcmgh.2022.11.008
DO - 10.1016/j.jcmgh.2022.11.008
M3 - Review article
C2 - 36442812
AN - SCOPUS:85146450317
SN - 2352-345X
VL - 15
SP - 559
EP - 571
JO - Cellular and Molecular Gastroenterology and Hepatology
JF - Cellular and Molecular Gastroenterology and Hepatology
IS - 3
ER -