In vitro evaluation of cytotoxicity of engineered metal oxide nanoparticles

Xiaoke Hu; Sean Cook; Peng Wang; Huey min Hwang

doi:10.1016/j.scitotenv.2009.01.033

In vitro evaluation of cytotoxicity of engineered metal oxide nanoparticles

Xiaoke Hu
, Sean Cook
, Peng Wang
, Huey min Hwang

Research output: Contribution to journal › Article › peer-review

318 Scopus citations

Abstract

The recent advances in nanotechnology and the corresponding popular usage of nanomaterials have resulted in uncertainties regarding their environmental impacts. In this study, we used a systematic approach to study and compare the in vitro cytotoxicity of selected engineered metal oxide nanoparticles to the test organisms - E. coli. Among the seven test nano-sized metal oxides, ZnO, CuO, Al₂O_3, La₂O_3, Fe₂O_3, SnO₂ and TiO_2, ZnO showed the lowest LD₅₀ of 21.1 mg/L and TiO₂ had the highest LD₅₀ of 1104.8 mg/L. Data of ¹⁴C-glucose mineralization test paralleled the results of bacteria viability test. After regression calculation, the cytotoxicity was found to be correlated with cation charges (R² = 0.9785). The higher the cation charge is, the lower the cytotoxicity of the nano-sized metal oxide becomes. To the best of our knowledge, this finding is the first report in nanotoxicology.

Original language	English
Pages (from-to)	3070-3072
Number of pages	3
Journal	Science of the Total Environment
Volume	407
Issue number	8
DOIs	https://doi.org/10.1016/j.scitotenv.2009.01.033
State	Published - 1 Apr 2009
Externally published	Yes

Keywords

Cytotoxicity
LD
Metal oxide nanoparticles

Access to Document

10.1016/j.scitotenv.2009.01.033

Cite this

@article{59789a9bcaca404bb409780864e236a7,

title = "In vitro evaluation of cytotoxicity of engineered metal oxide nanoparticles",

abstract = "The recent advances in nanotechnology and the corresponding popular usage of nanomaterials have resulted in uncertainties regarding their environmental impacts. In this study, we used a systematic approach to study and compare the in vitro cytotoxicity of selected engineered metal oxide nanoparticles to the test organisms - E. coli. Among the seven test nano-sized metal oxides, ZnO, CuO, Al2O3, La2O3, Fe2O3, SnO2 and TiO2, ZnO showed the lowest LD50 of 21.1 mg/L and TiO2 had the highest LD50 of 1104.8 mg/L. Data of 14C-glucose mineralization test paralleled the results of bacteria viability test. After regression calculation, the cytotoxicity was found to be correlated with cation charges (R2 = 0.9785). The higher the cation charge is, the lower the cytotoxicity of the nano-sized metal oxide becomes. To the best of our knowledge, this finding is the first report in nanotoxicology.",

keywords = "Cytotoxicity, LD, Metal oxide nanoparticles",

author = "Xiaoke Hu and Sean Cook and Peng Wang and Hwang, \{Huey min\}",

note = "Funding Information: This research was supported by the following grants: (1) U. S. Department of the Army Research and Development grant \#W912HZ-04-2-0002 to Jackson State University (JSU); (2) NSF CREST Program with grant \#HRD-0833178 for supporting Interdisciplinary Nanotoxicity Center; (3) and Department of Energy (DOE-MAT-SBI Program), grant \#DE-FG36-05G085002 with subcontract\# 07-08-001 to JSU.",

year = "2009",

month = apr,

day = "1",

doi = "10.1016/j.scitotenv.2009.01.033",

language = "English",

volume = "407",

pages = "3070--3072",

journal = "Science of the Total Environment",

issn = "0048-9697",

publisher = "Elsevier B.V.",

number = "8",

}

TY - JOUR

T1 - In vitro evaluation of cytotoxicity of engineered metal oxide nanoparticles

AU - Hu, Xiaoke

AU - Cook, Sean

AU - Wang, Peng

AU - Hwang, Huey min

N1 - Funding Information: This research was supported by the following grants: (1) U. S. Department of the Army Research and Development grant #W912HZ-04-2-0002 to Jackson State University (JSU); (2) NSF CREST Program with grant #HRD-0833178 for supporting Interdisciplinary Nanotoxicity Center; (3) and Department of Energy (DOE-MAT-SBI Program), grant #DE-FG36-05G085002 with subcontract# 07-08-001 to JSU.

PY - 2009/4/1

Y1 - 2009/4/1

N2 - The recent advances in nanotechnology and the corresponding popular usage of nanomaterials have resulted in uncertainties regarding their environmental impacts. In this study, we used a systematic approach to study and compare the in vitro cytotoxicity of selected engineered metal oxide nanoparticles to the test organisms - E. coli. Among the seven test nano-sized metal oxides, ZnO, CuO, Al2O3, La2O3, Fe2O3, SnO2 and TiO2, ZnO showed the lowest LD50 of 21.1 mg/L and TiO2 had the highest LD50 of 1104.8 mg/L. Data of 14C-glucose mineralization test paralleled the results of bacteria viability test. After regression calculation, the cytotoxicity was found to be correlated with cation charges (R2 = 0.9785). The higher the cation charge is, the lower the cytotoxicity of the nano-sized metal oxide becomes. To the best of our knowledge, this finding is the first report in nanotoxicology.

AB - The recent advances in nanotechnology and the corresponding popular usage of nanomaterials have resulted in uncertainties regarding their environmental impacts. In this study, we used a systematic approach to study and compare the in vitro cytotoxicity of selected engineered metal oxide nanoparticles to the test organisms - E. coli. Among the seven test nano-sized metal oxides, ZnO, CuO, Al2O3, La2O3, Fe2O3, SnO2 and TiO2, ZnO showed the lowest LD50 of 21.1 mg/L and TiO2 had the highest LD50 of 1104.8 mg/L. Data of 14C-glucose mineralization test paralleled the results of bacteria viability test. After regression calculation, the cytotoxicity was found to be correlated with cation charges (R2 = 0.9785). The higher the cation charge is, the lower the cytotoxicity of the nano-sized metal oxide becomes. To the best of our knowledge, this finding is the first report in nanotoxicology.

KW - Cytotoxicity

KW - LD

KW - Metal oxide nanoparticles

UR - https://www.scopus.com/pages/publications/61449116709

U2 - 10.1016/j.scitotenv.2009.01.033

DO - 10.1016/j.scitotenv.2009.01.033

M3 - Article

C2 - 19215968

AN - SCOPUS:61449116709

SN - 0048-9697

VL - 407

SP - 3070

EP - 3072

JO - Science of the Total Environment

JF - Science of the Total Environment

IS - 8

ER -

In vitro evaluation of cytotoxicity of engineered metal oxide nanoparticles

Abstract

Keywords

Access to Document

Fingerprint

Cite this