In Vitro Cytokinetic Response of Human Colon Cancer Cells to cis-Dichlorodiammineplatinum(ll)

Bergerat Jean-Pierre; Barthel Barlogie; Wolfgang Gohde; Dennis A. Johnston; Benjamin Drewinko

In Vitro Cytokinetic Response of Human Colon Cancer Cells to cis-Dichlorodiammineplatinum(ll)

Bergerat Jean-Pierre, Barthel Barlogie, Wolfgang Gohde, Dennis A. Johnston, Benjamin Drewinko

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

The cytokinetic response of a human colon carcinoma cell line to cis-dichlorodiammineplatinum(II) was investigated using flow cytometry of DNA content, autoradiography after pulse and continuous tritiated thymidine exposure, and mitotic accumulation after continuous Colcemid treatment. With increasing concentration and exposure time, c/s-dichlorodiamminepla-tinum(ll) delayed and then blocked cycle traverse in S and G2 phases. After prolonged treatment with high concentrations of cis-dichlorodiammineplatinum(II) an additional block in G1 or at the Gi-S boundary was established. Irreversibility of cell cycle distribution changes after prolonged observation periods suggests cell death in G2, S, and G1 compartments.

Original language	English
Pages (from-to)	4356-4363
Number of pages	8
Journal	Cancer Research
Volume	39
Issue number	11
State	Published - 1 Nov 1979
Externally published	Yes

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title = "In Vitro Cytokinetic Response of Human Colon Cancer Cells to cis-Dichlorodiammineplatinum(ll)",

abstract = "The cytokinetic response of a human colon carcinoma cell line to cis-dichlorodiammineplatinum(II) was investigated using flow cytometry of DNA content, autoradiography after pulse and continuous tritiated thymidine exposure, and mitotic accumulation after continuous Colcemid treatment. With increasing concentration and exposure time, c/s-dichlorodiamminepla-tinum(ll) delayed and then blocked cycle traverse in S and G2 phases. After prolonged treatment with high concentrations of cis-dichlorodiammineplatinum(II) an additional block in G1 or at the Gi-S boundary was established. Irreversibility of cell cycle distribution changes after prolonged observation periods suggests cell death in G2, S, and G1 compartments.",

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AU - Jean-Pierre, Bergerat

AU - Barlogie, Barthel

AU - Gohde, Wolfgang

AU - Johnston, Dennis A.

AU - Drewinko, Benjamin

PY - 1979/11/1

Y1 - 1979/11/1

N2 - The cytokinetic response of a human colon carcinoma cell line to cis-dichlorodiammineplatinum(II) was investigated using flow cytometry of DNA content, autoradiography after pulse and continuous tritiated thymidine exposure, and mitotic accumulation after continuous Colcemid treatment. With increasing concentration and exposure time, c/s-dichlorodiamminepla-tinum(ll) delayed and then blocked cycle traverse in S and G2 phases. After prolonged treatment with high concentrations of cis-dichlorodiammineplatinum(II) an additional block in G1 or at the Gi-S boundary was established. Irreversibility of cell cycle distribution changes after prolonged observation periods suggests cell death in G2, S, and G1 compartments.

AB - The cytokinetic response of a human colon carcinoma cell line to cis-dichlorodiammineplatinum(II) was investigated using flow cytometry of DNA content, autoradiography after pulse and continuous tritiated thymidine exposure, and mitotic accumulation after continuous Colcemid treatment. With increasing concentration and exposure time, c/s-dichlorodiamminepla-tinum(ll) delayed and then blocked cycle traverse in S and G2 phases. After prolonged treatment with high concentrations of cis-dichlorodiammineplatinum(II) an additional block in G1 or at the Gi-S boundary was established. Irreversibility of cell cycle distribution changes after prolonged observation periods suggests cell death in G2, S, and G1 compartments.

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JO - Cancer Research

JF - Cancer Research

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ER -

In Vitro Cytokinetic Response of Human Colon Cancer Cells to cis-Dichlorodiammineplatinum(ll)

Abstract

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