In Vitro cleavage of Nedd8 from cullin 1 by COP9 signalosome and deneddylase 1

Kosj Yamoah, Kenneth Wu, Zhen Qiang Pan

Research output: Contribution to journalReview articlepeer-review

12 Scopus citations

Abstract

Enzymatic cleavage of Nedd8 from its cullin conjugates plays a critical role in ubiquitin-dependent proteolysis by regulating the activity of the cullin-based RING-H2 E3 ubiquitin ligases. This chapter provides methods for the preparation of two Nedd8 isopeptidases: the COP9 signalosome and human deneddylase 1. It also describes the development of cell-free systems for cleavage of the Nedd8-cullin 1 isopeptide bond formed in vitro or in vivo.

Original languageEnglish
Pages (from-to)509-522
Number of pages14
JournalMethods in Enzymology
Volume398
DOIs
StatePublished - 2005

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