TY - JOUR
T1 - Improving nuclear morphometry imaging with real-time and low-cost line-scanning confocal microendoscope
AU - Tang, Yubo
AU - Kortum, Alex
AU - Vohra, Imran
AU - Othman, Mohamed
AU - Dhingra, Sadhna
AU - Mansour, Nabil
AU - Carns, Jennifer
AU - Anandasabapathy, Sharmila
AU - Richards-Kortum, Rebecca
N1 - Publisher Copyright:
© 2019 Optical Society of America
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Fiber-optic endomicroscopy is a minimally invasive tool to probe disease progression with subcellular resolution. In this Letter, we demonstrate a low-cost and compact fluorescence microendoscope capable of line-scanning confocal imaging by synchronizing a digital light projector with a CMOS camera. We present the digital aperture design to enable real-time confocal imaging, and we implement parallel illumination to improve the optical sectioning performance. Furthermore, we show that the confocal microendoscope can enhance visualization of disease-associated features when imaging highly scattering esophageal specimens.
AB - Fiber-optic endomicroscopy is a minimally invasive tool to probe disease progression with subcellular resolution. In this Letter, we demonstrate a low-cost and compact fluorescence microendoscope capable of line-scanning confocal imaging by synchronizing a digital light projector with a CMOS camera. We present the digital aperture design to enable real-time confocal imaging, and we implement parallel illumination to improve the optical sectioning performance. Furthermore, we show that the confocal microendoscope can enhance visualization of disease-associated features when imaging highly scattering esophageal specimens.
UR - https://www.scopus.com/pages/publications/85060951199
U2 - 10.1364/OL.44.000654
DO - 10.1364/OL.44.000654
M3 - Article
C2 - 30702702
AN - SCOPUS:85060951199
SN - 0146-9592
VL - 44
SP - 654
EP - 657
JO - Optics Letters
JF - Optics Letters
IS - 3
ER -