TY - JOUR
T1 - Improved identification of von Hippel-Lindau gene alterations in clear cell renal tumors
AU - Nickerson, Michael L.
AU - Jaeger, Erich
AU - Shi, Yangu
AU - Durocher, Jeffrey A.
AU - Mahurkar, Sunil
AU - Zaridze, David
AU - Matveev, Vsevolod
AU - Janout, Vladimir
AU - Kollarova, Hellena
AU - Bencko, Vladimir
AU - Navratilova, Marie
AU - Szeszenia-Dabrowska, Neonilia
AU - Mates, Dana
AU - Mukeria, Anush
AU - Holcatova, Ivana
AU - Schmidt, Laura S.
AU - Toro, Jorge R.
AU - Karami, Sara
AU - Hung, Rayjean
AU - Gerard, Gary F.
AU - Linehan, W. Marston
AU - Merino, Maria
AU - Zbar, Berton
AU - Boffetta, Paolo
AU - Brennan, Paul
AU - Rothman, Nathaniel
AU - Chow, Wong Ho
AU - Waldman, Frederic M.
AU - Moore, Lee E.
PY - 2008/8/1
Y1 - 2008/8/1
N2 - Purpose: To provide a comprehensive, thorough analysis of somatic mutation and promoter hypermethylation of the von Hippel-Lindau {VHL) gene in the cancer genome, unique to clear cell renal cancer (ccRCC). Identify relationships between the prevalence of VHL gene alterations and alteration subtypes with patient and tumor characteristics. Experimental Design: As part of a large kidney cancer case-control study conducted in Central Europe, we analyzed VHL mutations and promoter methylation in 205 well-characterized, histologically confirmed patient tumor biopsies using a combination of sensitive, high-throughput methods (endonuclease scanning and Sanger sequencing) and analysis of 11 CpG sites in the VHL promoter. Results: We identified mutations in 82.4% of cases, the highest VHL gene mutation prevalence reported to date. Analysis of 11 VHL promoter CpG sites revealed that 8.3% of tumors were hypermethylated and all were mutation negative. In total, 91% of ccRCCs exhibited alteration of the gene through genetic or epigenetic mechanisms. Analysis of patient and tumor characteristics revealed that certain mutation subtypes were significantly associated with Fuhrman nuclear grade, metastasis, node positivity, and self-reported family history of RCC, Conclusion: Detection of VHL gene alterations using these accurate, sensitive, and practical methods provides evidence that the vast majority of histologically confirmed ccRCC tumors possess genetic or epigenetic alteration of the VHL gene and support the hypothesis that VHL alteration is an early event in ccRCC carcinogenesis. These findings also indicate that VHL molecular subtypes can provide a sensitive marker of tumor heterogeneity among histologically similar ccRCC cases for etiologic, prognostic, and translational studies.
AB - Purpose: To provide a comprehensive, thorough analysis of somatic mutation and promoter hypermethylation of the von Hippel-Lindau {VHL) gene in the cancer genome, unique to clear cell renal cancer (ccRCC). Identify relationships between the prevalence of VHL gene alterations and alteration subtypes with patient and tumor characteristics. Experimental Design: As part of a large kidney cancer case-control study conducted in Central Europe, we analyzed VHL mutations and promoter methylation in 205 well-characterized, histologically confirmed patient tumor biopsies using a combination of sensitive, high-throughput methods (endonuclease scanning and Sanger sequencing) and analysis of 11 CpG sites in the VHL promoter. Results: We identified mutations in 82.4% of cases, the highest VHL gene mutation prevalence reported to date. Analysis of 11 VHL promoter CpG sites revealed that 8.3% of tumors were hypermethylated and all were mutation negative. In total, 91% of ccRCCs exhibited alteration of the gene through genetic or epigenetic mechanisms. Analysis of patient and tumor characteristics revealed that certain mutation subtypes were significantly associated with Fuhrman nuclear grade, metastasis, node positivity, and self-reported family history of RCC, Conclusion: Detection of VHL gene alterations using these accurate, sensitive, and practical methods provides evidence that the vast majority of histologically confirmed ccRCC tumors possess genetic or epigenetic alteration of the VHL gene and support the hypothesis that VHL alteration is an early event in ccRCC carcinogenesis. These findings also indicate that VHL molecular subtypes can provide a sensitive marker of tumor heterogeneity among histologically similar ccRCC cases for etiologic, prognostic, and translational studies.
UR - http://www.scopus.com/inward/record.url?scp=51049084870&partnerID=8YFLogxK
U2 - 10.1158/1078-0432.CCR-07-4921
DO - 10.1158/1078-0432.CCR-07-4921
M3 - Article
C2 - 18676741
AN - SCOPUS:51049084870
SN - 1078-0432
VL - 14
SP - 4726
EP - 4734
JO - Clinical Cancer Research
JF - Clinical Cancer Research
IS - 15
ER -