Impacto de las lesiones por isquemia-reperfusión en la supervivencia alejada de ratas con trasplante intestinal

Translated title of the contribution: Impact of ishemia-reperfusion injury on long survival rate in intestinal transplantation in rats

Natalia Lausada, Pablo Stringa, Ana Cabanne, Diego Ramisch, Mariana Machuca, Flavio Galvao, Silvia Coronato, Jorge Clemente Raimondi, Gabriel Gondolesi

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Introduction. The intestine is a highly sensitive tissue to ischemia-reperfusion (IR) injury that will early respond increasing its permeability. Later this response is translated in morphologic and histological changes that reveal the degree of damage. The heterotopic intestinal transplantation model in rats allows to evaluate the evolution of intestinal tissue injury after ischemiareperfusion without affecting the long survival rate. Objective. The aim of this paper is to establish a relationship between the ischemic reperfusion injury with the long-term survival. Methods. Ten intestinal transplants were analyzed in adult, Wistar, inbred, male rats. Light microscopical examination was performed on intestine graft: 1) immediately post-dissection, 2) at the end of cold isquemia, 3) 30 min, 4) 48hs and 5) 5 days post-transplant procedure, respectively. Biopsies were reported according to Park's classification and extension of staining using immunohistochemestry to malondialdehyde (MDA) products. Results. The Park's classification indexes reported in samples were 1) 0,57±1,13 (N=10); 2) 2,71±1,25 (N=10); 3) 4,14±0,89 (N=10); 4) 1,0±0,81 (N=7); 5) 0 (N=7). The highest levels of immunohistochemical detection of MDA were observed thirty minutes post-reperfusion (extension of staining between 51% to 75%). Three animals died when they were sampled at 48 hours, and the biopsies had Park's classification ≥4 at 30minutes post-reperfusion and endotoxemic signology. Conclusions. The highest degree of mucosal damage was observed immediately post-reperfusión. At 48hs the graft tended to be normalized. Failure to repair the immediately I-R injury significantly affects the long term survival.

Translated title of the contributionImpact of ishemia-reperfusion injury on long survival rate in intestinal transplantation in rats
Original languageSpanish
Pages (from-to)129-136
Number of pages8
JournalActa Gastroenterologica Latinoamericana
Volume41
Issue number2
StatePublished - Jun 2011
Externally publishedYes

Keywords

  • Ischemia-reperfusion injury
  • Isogeneic/methods intestines/transplantation
  • Pathology
  • Rats

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