Impact of using validated or standard reference genes for miRNA qPCR data normalization in cell type identification

M. Sirker, W. Liang, L. Zhang, R. Fimmers, M. A. Rothschild, I. Gomes, P. M. Schneider

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

For the analysis of cell type-specific miRNA expression patterns qPCR is currently the method of choice owing to its high accuracy. However, to obtain reliable results, a proper normalization strategy is an absolute prerequisite, which is often underestimated. To demonstrate the importance of using a set of suitable reference genes, we tested two normalization strategies by comparing gene expressions of tissue-specific miRNA targets normalized against: (1) previously validated endogenous controls (miR92 and miR374) and (2) a commonly used miRNA reference (U6B).

Original languageEnglish
Pages (from-to)e199-e201
JournalForensic Science International: Genetics Supplement Series
Volume5
DOIs
StatePublished - 1 Dec 2015
Externally publishedYes

Keywords

  • Body fluid identification
  • Forensic science
  • GeNorm
  • Normalization
  • Reference genes
  • miRNA profiling

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