TY - JOUR
T1 - Impact of α-defensins1-3 on the maturation and differentiation of human monocyte-derived DCs. Concentration-dependent opposite dual effects
AU - Rodríguez-García, Marta
AU - Oliva, Harold
AU - Climent, Núria
AU - Escribese, Maria M.
AU - García, Felipe
AU - Moran, Thomas M.
AU - Gatell, José M.
AU - Gallart, Teresa
N1 - Funding Information:
M R-G is the recipient of a research award “Emili Letang” (Hospital Clínic, Barcelona). This study was supported mainly by Research Grants FIS03-1200 and FIS2006-1259 (T. G.) from the Spanish Ministry of Health, and by the Research Grant NIH NO1-AI-50028 (T.M.M). Additional support was also received from the research grants SAF2005-05566 (J. M. G.) from the Spanish Ministry of Education and Science, FIPSE36536-2005 (T. G.), the Foundation for the Investigation and Prevention of AIDS in Spain, and ISCIII-RETIC RD06/006 from the Spanish Cooperative Network of AIDS Research Groups from the Ministry of Health. “Fundacion Máximo Soriano Jiménez” also contributed (HO). We thank C. Rovira and L. Miralles for their invaluable technical assistance.
PY - 2009/6
Y1 - 2009/6
N2 - α-defensins1-3 are potent antimicrobial molecules that also link innate and adaptive immunity, depending on the concentration range. However, their effects on the biology of human DCs remain largely unknown. We analyzed the impact of different concentrations of α-defensins1-3 on the maturation and differentiation of monocyte-derived DCs (MDDCs). Low doses of α-defensins1-3 up-regulated CD83, CD86 and HLA-DR expression, increased TNF-α, IL-1β, IL-12p40, IL-10 and IL-8 secretion, and slightly augmented allostimulatory capacity. By contrast, high doses down-regulated CD86 and HLA-DR expression, TNF-α, IL-1β, IL-12p40 and IL-10 secretion and allostimulatory capacity, whereas strongly up-regulated IL-8. Furthermore, during the MDDC differentiation process, high doses of α-defensins1-3 affected CD14, CD11c and CD86 expression and strongly up-regulated IL-8. Results suggest that α-defensins1-3 might modulate the maturation and differentiation of MDDCs in vivo and therefore could be of special interest in the field of vaccine development.
AB - α-defensins1-3 are potent antimicrobial molecules that also link innate and adaptive immunity, depending on the concentration range. However, their effects on the biology of human DCs remain largely unknown. We analyzed the impact of different concentrations of α-defensins1-3 on the maturation and differentiation of monocyte-derived DCs (MDDCs). Low doses of α-defensins1-3 up-regulated CD83, CD86 and HLA-DR expression, increased TNF-α, IL-1β, IL-12p40, IL-10 and IL-8 secretion, and slightly augmented allostimulatory capacity. By contrast, high doses down-regulated CD86 and HLA-DR expression, TNF-α, IL-1β, IL-12p40 and IL-10 secretion and allostimulatory capacity, whereas strongly up-regulated IL-8. Furthermore, during the MDDC differentiation process, high doses of α-defensins1-3 affected CD14, CD11c and CD86 expression and strongly up-regulated IL-8. Results suggest that α-defensins1-3 might modulate the maturation and differentiation of MDDCs in vivo and therefore could be of special interest in the field of vaccine development.
KW - Antimicrobial peptides
KW - Cytokines
KW - Dendritic cells
KW - Human neutrophil peptides1-3
KW - Innate immunity
UR - https://www.scopus.com/pages/publications/67349200264
U2 - 10.1016/j.clim.2009.01.012
DO - 10.1016/j.clim.2009.01.012
M3 - Article
C2 - 19237318
AN - SCOPUS:67349200264
SN - 1521-6616
VL - 131
SP - 374
EP - 384
JO - Clinical Immunology
JF - Clinical Immunology
IS - 3
ER -