Immunoperoxidase technique for detecting spirochetes in tissue sections: Comparison with other methods

Robert George Phelps, Jeffrey Knispel, Erik Schuman Tu, Georgetta Cernainu, Michael Saruk

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Background: With the increasing incidence of human immunodeficiency virus (HIV) infection and immunosuppressive therapy, the incidence of syphilis has been increasing. Given the fact that the above conditions may mask or obscure the usual clinical signs and symptoms of syphilis, a means of enhanced detection is essential. Aims and methods: The purpose of this study was to determine whether an immuno-peroxidase method using an antibody against treponemes would increase the sensitivity and specificity of diagnosis in biopsies of patients with secondary syphilis. This was compared to serology and silver stain in cases of known syphilis. Results: Immunoperoxidase for treponemes was at least as sensitive (9/10) as pathology (9/10), and more sensitive than conventional silver stain (6/10) or serology (7/10). Conclusions: In those equivocal cases of secondary syphilis, where confirmation is essential, immunoperoxidase for treponemes may be a useful adjunct.

Original languageEnglish
Pages (from-to)609-613
Number of pages5
JournalInternational Journal of Dermatology
Volume39
Issue number8
DOIs
StatePublished - 2000

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