Immunohistochemical localization of modified C-reactive protein antigen in normal vascular tissue

Edward E. Diehl, G. Kenneth Haines, James A. Radosevich, Lawrence A. Potempa

Research output: Contribution to journalArticlepeer-review

103 Scopus citations

Abstract

Background: The prototypic acute phase reactant, C-reactive protein (CRP), is a serum soluble, cyclic pentameric protein, the concentration of which increases markedly within hours of any tissue-damaging, inflammatory event. However, upon dissociation of its pentameric quaternary structure, CRP subunits undergo a spontaneous and irreversible conformational change. The resulting molecule, termed modified CRP or mCRP, has reduced aqueous solubility and a propensity to aggregate into a matrix-like lattice structure. Methods: Using monoclonal antibodies, normal human tissues were immunohistochemically screened for the presence of CRP as well as mCRP antigens. Results: Significant levels of mCRP were detected in the walls of blood vessels associated with normal human tissues. These data indicate that mCRP is a naturally occurring form of CRP and that it is a tissue-based rather than serum-based molecule. Significance: This report describes the localization of a stable form of CRP, mCRP, in blood vessels associated with normal human tissues.

Original languageEnglish
Pages (from-to)79-83
Number of pages5
JournalAmerican Journal of the Medical Sciences
Volume319
Issue number2
DOIs
StatePublished - Feb 2000
Externally publishedYes

Keywords

  • Blood vessel
  • C-reactive protein (CRP)
  • Immunohistochemistry
  • Intima
  • Modified C-reactive protein (mCRP)

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