Immunohistochemical detection of epitopes expressed on cRP and modified-CRP (i.e. NEO-CRP) in human normal and diseased tissues

J. A. Radosevich, G. K. Haines, M. Motie, K. W. Schaul, N. Mehta, K. Kolb, L. A. Potempa

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

C-reactive protein (CRP) can exist in a second, conformationally distinct form (i.e. modified-CRP) which expresses unique biochemical and antigenic (i.e. neo-CRP) characteristics. Using specific mAbs to each form of CRP, over 60 fresh, unfixed normal and diseased human tissues were collected, frozen-sectioned and stained using immunoperoxidase techniques. CRP epitopes were not consistently expressed in any tissue, occasionally being noted at the tunica media of blood vessels. In contrast, specific neo-CRP epitopes were abundantly expressed at the intima, and in the media and adventitia of a large number of normal vessels. This immunoreactivity was also found in the fibrous capsules of the adrenal gland and tonsil, the fibrous trabeculae of spleen, and in skin and skeletal muscle. However, neo-CRP immunoreactivity was not expressed, or was expressed at reduced levels, in a variety of cancer specimens. These data suggest a protein antigenically related to mCRP is expressed in certain normal fibrous tissues. Reduced expression of this antigen in disease tissue may have clinical significance.

Original languageEnglish
Pages (from-to)A1466
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996
Externally publishedYes

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