Immunohistochemical detection of double-stranded-RNA-dependent protein kinase (p68) with a novel monoclonal antibody TJ4C4

G. K. Haines, G. D. Ghadge, S. G. Combs, W. Leslie, B. Thimmappaya, James A. Radosevich

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Double-stranded RNA (dsRNA)-dependent protein kinase (p68) has been shown to be induced by alpha-interferon (IFN-α) in mammalian cells. It binds to dsRNA, and is believed to be a factor in the control of both cellular and viral protein synthesis. This report describes the use of a new monoclonal antibody (MAb) TJ4C4, to monitor levels of p68 in a patient with AIDS-associated Kaposi’s sarcoma. Using a novel immu-noperoxidase/iron staining method, we examined formalin-fixed, paraffin-embedded biopsies prior to, and 4 months after the initiation of IFN therapy. Immunostaining showed low levels (1+ staining) of p68 in the pretreatment tissue, whereas a marked increase (4+ staining) was noted during interferon treatment. This staining suggests an increased level of intracellular p68 expression. This patient has subsequently remained on IFN-α therapy and is alive with no evidence of Kaposi’s sarcoma, 6 1/2 years after diagnosis. The use of MAb TJ4C4 will greatly facilitate the study of p68 kinase in clinical tissues, and may provide a way to monitor the effects of IFN therapy.

Original languageEnglish
Pages (from-to)324-329
Number of pages6
JournalTumor Biology
Volume13
Issue number5-6
DOIs
StatePublished - 1992
Externally publishedYes

Keywords

  • AIDS
  • Immunoperoxidase/iron stain
  • Interferon-α
  • Kaposi’s sarcoma
  • MAb TJ4C4
  • Sarcoma
  • eIF-2a kinase
  • p68

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