TY - JOUR
T1 - Immunoglobulin synthesis and glucose-6-phosphate dehydrogenase as cell markers in human lymphoblastoid cell lines
AU - Béchet, J. M.
AU - Fialkow, P. J.
AU - Nilsson, K.
AU - Klein, G.
AU - Singh, S.
N1 - Funding Information:
had type A enzymew hile other lines from the averagen umbero f heavya nd light chain the same tonsil had type B, argues against typess ynthesizedb y eachl ine was lower than this possibility. (2) Some lines may have in the tonsil-derivedli nes. This suggeststh at originated from several cells differing by the numbero f progenitorc ellsf or tonsil LCL their immunoglobulins ynthesis,b ut having is greatert han for lymph node LCL. There by chancet he sameG -6-PD phenotypeS. uch are severalp ossibilitiest o explain this differ-a coincidencein almost everyl ine, however, ence:( 1) the tonsil biopsiesw ere larger and is unlikely. (3) The phenomenonw hich deter-this alloweda greatera mounto f materialt o minesG -6-PD genei nactivationo ccurse arly be seededi n each culture; (2) the chronic in embryogenesiasn d is fixed. Thus, a line inflammationi n the tonsils may have poten-may start from a single immaturec ell with tiated EBV transformation; (3) the only only one G-6-PD genea ctive,b ut still pluri-known site of productive EBV infection of potential for immunoglobulins ynthesis.( 4) cells with releaseo f infectiousv irus particles The techniquesfo r assayingt he two markers is the oropharyngeala rea [14, 221. Thus, may differ in sensitivity.I n order to detect tonsils may have more EBV or EBV-trans-a minor cell componentw ith the G-6-PD formedc ells than other lymphoid tissues. system,i t probably must contributea t least SomeB urkitt lines in this and other studies 510% of the total enzymea ctivity in the did not secretea ny Ig (table 1) [9, 13,2 4, 301. sample. The sensitivity of the immunoglo-In contrast,a ll of the tonsil lines (table 2) bulin assayw as not quantitatedb, ut it might and almosta ll previousL CL establishedfr om have detectedi mmunoglobulinss ynthesized non-Burkitt neoplasmsa nd from non-malig-by a muchs mallerf ractiono f the cell popula-nant lymphoid tissuesh aves ecretedIg [3,23]. tion. The absenceo f Ig secretionin someB urkitt In severalli nes an immunoglobulinc ompo-LCL and of cell surface-associateIdg in nent that was not detectede arly after estab-manyB urkitt tumours[ 7]r aiset he possibility lishment,w asf ound at a later time.A lthough that some Burkitt neoplasmsa re of T cell the possibilityt hat thesel ines werea ccident-origin. However,s ince it is conceivablet hat ally contaminatedin vitro cannotb e excluded, malignantt ransformationo f a T or B cell another possibility is that cells presenti ni-may change its Ig-synthesizingf unctions, tially as minor populations subsequently these criteria alone should not be used to becamem ajor componentso f the lines. classifyt umoursa s B or T cell in origin. Almost all LCL establishedfr om Burkitt lymphomas and non-neoplastic lymphoid Marianne Petterssona nd Gabriel Herner is gratefully The skilful technical assistanceo f Annika Jordell, tissues carry the Epstein-Barr virus (EBV) acknowledged. genome [16]. The evidencep resentedh ere the King Gustav V Jubilee Fund and 72:213 from This work was supported by grants 70: 23 from for multicellular origin of lymphoid lines the Swedish Cancer Society. bv a research training derivedf rom non-neoplastict onsils suggests search on Cancer and by Grants GM 15253 and fellowship from the International Agency for Re- that the type of EBV transformationw hich 5 ROl CA 14054-02f rom the NIH of the USPHS. endowsa cell with the capacityt o proliferate J. M. B. is attache de recherchesa I’INSERM. indefinitely in vitro may be a not-so-rare event. Heterogeneoupsa tternso f immunoglobulin synthesisw ere previously observedi n lines derivedf rom lymph nodes [3, 231.H owever,
PY - 1974/12
Y1 - 1974/12
N2 - Multiple lymphoblastoid cell lines were established from each of seven Burkitt lymphoma biopsies and from tonsils, removed from four patients with chronic tonsillitis. The cellular origin of the lines was studied using as markers the pattern of immunoglobulins secreted into the medium and the cells' glucose-6-phosphate dehydrogenase (G-6-PD) phenotypes. Lines from the same tonsil biopsy differed from each other by their patterns of immunoglobulin synthesis and G-6-PD phenotypes. All tonsil-derived lines secreted complete immunoglobulins. Newly established lines usually produced several heavy and light chain types, indicating multicellular origin, but the number of components produced decreased during the course of long-term cultivation. G-6-PD phenotypes of lines established from the same tonsil removed from a G-6-PD heterozygote differed-B, A and B/A phenotypes were found. The B/A lines rapidly changed to a single enzyme phenotype (B or A) when maintained in culture. The immunoglobulin and G-6-PD phenotypes in lines derived from Burkitt lymphomas differed from those of tonsil lines in several respects: (1) Some lines produced no immunoglobulins; (2) in immunoglobulin-synthesizing lines, the patterns of heavy and light chain production were more restricted than in tonsil lines; (3) after some months in culture, a uniform pattern of immunoglobulin synthesis was found in all lines derived from the same tumour; (4) lines from G-6-PD heterozygotes had the same single enzyme phenotypes as were found in the tumours. The data strongly suggest that most lines from Burkitt lymphomas are derived from the tumour clones and that most tonsil-derived lines have multicellular origin.
AB - Multiple lymphoblastoid cell lines were established from each of seven Burkitt lymphoma biopsies and from tonsils, removed from four patients with chronic tonsillitis. The cellular origin of the lines was studied using as markers the pattern of immunoglobulins secreted into the medium and the cells' glucose-6-phosphate dehydrogenase (G-6-PD) phenotypes. Lines from the same tonsil biopsy differed from each other by their patterns of immunoglobulin synthesis and G-6-PD phenotypes. All tonsil-derived lines secreted complete immunoglobulins. Newly established lines usually produced several heavy and light chain types, indicating multicellular origin, but the number of components produced decreased during the course of long-term cultivation. G-6-PD phenotypes of lines established from the same tonsil removed from a G-6-PD heterozygote differed-B, A and B/A phenotypes were found. The B/A lines rapidly changed to a single enzyme phenotype (B or A) when maintained in culture. The immunoglobulin and G-6-PD phenotypes in lines derived from Burkitt lymphomas differed from those of tonsil lines in several respects: (1) Some lines produced no immunoglobulins; (2) in immunoglobulin-synthesizing lines, the patterns of heavy and light chain production were more restricted than in tonsil lines; (3) after some months in culture, a uniform pattern of immunoglobulin synthesis was found in all lines derived from the same tumour; (4) lines from G-6-PD heterozygotes had the same single enzyme phenotypes as were found in the tumours. The data strongly suggest that most lines from Burkitt lymphomas are derived from the tumour clones and that most tonsil-derived lines have multicellular origin.
UR - https://www.scopus.com/pages/publications/0016314290
U2 - 10.1016/0014-4827(74)90791-5
DO - 10.1016/0014-4827(74)90791-5
M3 - Article
C2 - 4218170
AN - SCOPUS:0016314290
SN - 0014-4827
VL - 89
SP - 275
EP - 282
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -