Immunoglobulin A Targets a Unique Subset of the Microbiota in Inflammatory Bowel Disease

Jason M. Shapiro, Marcel R. de Zoete, Noah W. Palm, Yaro Laenen, Rene Bright, Meaghan Mallette, Kevin Bu, Agata A. Bielecka, Fang Xu, Andres Hurtado-Lorenzo, Samir A. Shah, Judy H. Cho, Neal S. LeLeiko, Bruce E. Sands, Richard A. Flavell, J. C. Clemente

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


The immunopathogenesis of inflammatory bowel disease (IBD) has been attributed to a combination of host genetics and intestinal dysbiosis. Previous work in a small cohort of IBD patients suggested that pro-inflammatory bacterial taxa are highly coated with secretory immunoglobulin IgA. Using bacterial fluorescence-activated cell sorting coupled with 16S rRNA gene sequencing (IgA-SEQ), we profiled IgA coating of intestinal microbiota in a large cohort of IBD patients and identified bacteria associated with disease and treatment. Forty-three bacterial taxa displayed significantly higher IgA coating in IBD compared with controls, including 8 taxa exhibiting differential IgA coating but similar relative abundance. Patients treated with anti-TNF-α therapies exhibited dramatically altered microbiota-specific IgA responses compared with controls. Furthermore, increased IgA coating of Oscillospira was associated with a delay in time to surgery. These results demonstrate that investigating IgA responses to microbiota can uncover potential disease-modifying taxa and reveal improved biomarkers of clinical course in IBD.

Original languageEnglish
Pages (from-to)83-93.e3
JournalCell Host and Microbe
Issue number1
StatePublished - 13 Jan 2021


  • Crohn's disease
  • dysbiosis
  • immunoglobulin A
  • inflammatory bowel disease
  • microbiome
  • ulcerative colitis


Dive into the research topics of 'Immunoglobulin A Targets a Unique Subset of the Microbiota in Inflammatory Bowel Disease'. Together they form a unique fingerprint.

Cite this