Abstract
Immunofluorescent anti-tubulin staining has been used to follow nuclear progression from dictyate to metaphase II during meiotic maturation of mouse oocytes in vitro. Antibody directed against tubulin isolated from sea-urchin eggs decorates the metaphase I and metaphase II spindles, as well as the cytoplasmic bridge, midbody, and polar body of the maturing oocyte. Changes in the tubulin-specific staining pattern during meiotic maturation in vitro take place in a highly reproducible manner. Oocytes exposed continuously to cytochalasin B arrest at metaphase I and display a spindle which by immunofluorescent staining is virtually indistinguishable from the spindle of untreated oocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 171-188 |
| Number of pages | 18 |
| Journal | Journal of Cell Science |
| Volume | Vol. 29 |
| State | Published - 1978 |
| Externally published | Yes |