TY - JOUR
T1 - Immunoelectron microscopic localization of the F1F0 ATPase (ATP synthase) on the cytoplasmic membrane of alkalophilic Bacillus firmus RAB
AU - Rohde, Manfred
AU - Mayer, Frank
AU - Hicks, David B.
AU - Krulwich, Terry A.
N1 - Funding Information:
The authors are indebte.d to Dr. Nathan Nelson, Roche Institute of Molecular Biology for the generous gift of the anti-t-antibody. This work was supported by grants from the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie, Frankfurt, F.R.G. to F.M. and GM 28454 from the National Institute of General Medical Sciences to T.A.K. We thank U. Furstenau and C. Steinau for excellent technical assistance.
PY - 1989/10/16
Y1 - 1989/10/16
N2 - Evidence that the F1F0 ATPase (ATP synthase) of alkalophilic Bacillus firmus RAB is localized exclusively on the cytoplasmic membrane was obtained by immunogold electron microscopy using a highly specific polyclonal antibody against the β subunit of Escherichia coli F1F0 ATPase. The energetic problem faced by cells of B. firmus RAB growing oxidatively at pH 10.5 despite a low protonmotive force across the cytoplasmic membrane cannot, therefore, be circumvented by localization of energy transducing functions on hypothetical internal membranes.
AB - Evidence that the F1F0 ATPase (ATP synthase) of alkalophilic Bacillus firmus RAB is localized exclusively on the cytoplasmic membrane was obtained by immunogold electron microscopy using a highly specific polyclonal antibody against the β subunit of Escherichia coli F1F0 ATPase. The energetic problem faced by cells of B. firmus RAB growing oxidatively at pH 10.5 despite a low protonmotive force across the cytoplasmic membrane cannot, therefore, be circumvented by localization of energy transducing functions on hypothetical internal membranes.
UR - http://www.scopus.com/inward/record.url?scp=0024441979&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(89)90369-6
DO - 10.1016/0005-2736(89)90369-6
M3 - Article
C2 - 2529907
AN - SCOPUS:0024441979
SN - 0005-2736
VL - 985
SP - 233
EP - 235
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 2
ER -