Immunocytochemical localization of non-NMDA ionotropic excitatory amino acid receptor subunits in human neocortex

James C. Vickers; George W. Huntley; Patrick R. Hof; Joshua Bederson; Javier DeFelipe; John H. Morrison

doi:10.1016/0006-8993(94)01372-O

Immunocytochemical localization of non-NMDA ionotropic excitatory amino acid receptor subunits in human neocortex

James C. Vickers, George W. Huntley, Patrick R. Hof, Joshua Bederson, Javier DeFelipe, John H. Morrison

Research output: Contribution to journal › Article › peer-review

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Abstract

The distribution of immunocytochemically localized subunits that comprise ionotropic non-NMDA excitatory amino acid receptors was examined in human frontal, parietal and temporal association neocortex. AMPA/kainate receptor subunits were identified using a monoclonal antibody (3A11) that recognizes an epitope common to GluR2 and GluR4 [GluR2(4)], as well as polyclonal antisera that recognize GluR2 and GluR3 (GluR2/3). Kainate receptor subunits were identified using a monoclonal antibody (4F5) that recognizes an epitope common to GluR5/6/7. For all three antibodies used, labeling was observed in a large number of neurons throughout the human association neocortex with the highest immunoreactivity present in pyramidal-like neurons, a cellular pattern largely similar to that observed in the monkey neocortex. These data demonstrate the cellular localization patterns for some non-NMDA receptor subunits in human neocortex, details upon which further studies on the roles of these subunits in human neurological diseases can be based.

Original language	English
Pages (from-to)	175-180
Number of pages	6
Journal	Brain Research
Volume	671
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(94)01372-O
State	Published - 6 Feb 1995

Keywords

AMPA/kainate receptor
Association neocortex
Glutamate receptor
Human brain
Pyramidal neuron

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10.1016/0006-8993(94)01372-O

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title = "Immunocytochemical localization of non-NMDA ionotropic excitatory amino acid receptor subunits in human neocortex",

abstract = "The distribution of immunocytochemically localized subunits that comprise ionotropic non-NMDA excitatory amino acid receptors was examined in human frontal, parietal and temporal association neocortex. AMPA/kainate receptor subunits were identified using a monoclonal antibody (3A11) that recognizes an epitope common to GluR2 and GluR4 [GluR2(4)], as well as polyclonal antisera that recognize GluR2 and GluR3 (GluR2/3). Kainate receptor subunits were identified using a monoclonal antibody (4F5) that recognizes an epitope common to GluR5/6/7. For all three antibodies used, labeling was observed in a large number of neurons throughout the human association neocortex with the highest immunoreactivity present in pyramidal-like neurons, a cellular pattern largely similar to that observed in the monkey neocortex. These data demonstrate the cellular localization patterns for some non-NMDA receptor subunits in human neocortex, details upon which further studies on the roles of these subunits in human neurological diseases can be based.",

keywords = "AMPA/kainate receptor, Association neocortex, Glutamate receptor, Human brain, Pyramidal neuron",

author = "Vickers, {James C.} and Huntley, {George W.} and Hof, {Patrick R.} and Joshua Bederson and Javier DeFelipe and Morrison, {John H.}",

note = "Funding Information: This work was supportebdy NIH Grant AG05138, the DANA Foundationt,h eAaron DiamondF ounda-tion,t heA lexandrinaen dA lexandeSr insheimeFru nd, the BrookdaleF oundatioann dby FIS Grant9 4/0327. J.C.V. is a recipiento f a C.J. Martin Postdoctoral Fellowshipf rom the AustralianN ationalH ealthand MedicalR esearchC ouncil,G .W.H. is an Aaron Dia-mondF oundationFe llow,and P.R.H. is a Brookdale",

year = "1995",

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doi = "10.1016/0006-8993(94)01372-O",

language = "English",

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AU - Vickers, James C.

AU - Huntley, George W.

AU - Hof, Patrick R.

AU - Bederson, Joshua

AU - DeFelipe, Javier

AU - Morrison, John H.

N1 - Funding Information: This work was supportebdy NIH Grant AG05138, the DANA Foundationt,h eAaron DiamondF ounda-tion,t heA lexandrinaen dA lexandeSr insheimeFru nd, the BrookdaleF oundatioann dby FIS Grant9 4/0327. J.C.V. is a recipiento f a C.J. Martin Postdoctoral Fellowshipf rom the AustralianN ationalH ealthand MedicalR esearchC ouncil,G .W.H. is an Aaron Dia-mondF oundationFe llow,and P.R.H. is a Brookdale

PY - 1995/2/6

Y1 - 1995/2/6

N2 - The distribution of immunocytochemically localized subunits that comprise ionotropic non-NMDA excitatory amino acid receptors was examined in human frontal, parietal and temporal association neocortex. AMPA/kainate receptor subunits were identified using a monoclonal antibody (3A11) that recognizes an epitope common to GluR2 and GluR4 [GluR2(4)], as well as polyclonal antisera that recognize GluR2 and GluR3 (GluR2/3). Kainate receptor subunits were identified using a monoclonal antibody (4F5) that recognizes an epitope common to GluR5/6/7. For all three antibodies used, labeling was observed in a large number of neurons throughout the human association neocortex with the highest immunoreactivity present in pyramidal-like neurons, a cellular pattern largely similar to that observed in the monkey neocortex. These data demonstrate the cellular localization patterns for some non-NMDA receptor subunits in human neocortex, details upon which further studies on the roles of these subunits in human neurological diseases can be based.

AB - The distribution of immunocytochemically localized subunits that comprise ionotropic non-NMDA excitatory amino acid receptors was examined in human frontal, parietal and temporal association neocortex. AMPA/kainate receptor subunits were identified using a monoclonal antibody (3A11) that recognizes an epitope common to GluR2 and GluR4 [GluR2(4)], as well as polyclonal antisera that recognize GluR2 and GluR3 (GluR2/3). Kainate receptor subunits were identified using a monoclonal antibody (4F5) that recognizes an epitope common to GluR5/6/7. For all three antibodies used, labeling was observed in a large number of neurons throughout the human association neocortex with the highest immunoreactivity present in pyramidal-like neurons, a cellular pattern largely similar to that observed in the monkey neocortex. These data demonstrate the cellular localization patterns for some non-NMDA receptor subunits in human neocortex, details upon which further studies on the roles of these subunits in human neurological diseases can be based.

KW - AMPA/kainate receptor

KW - Association neocortex

KW - Glutamate receptor

KW - Human brain

KW - Pyramidal neuron

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JF - Brain Research

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ER -

Immunocytochemical localization of non-NMDA ionotropic excitatory amino acid receptor subunits in human neocortex

Abstract

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