Immunochemically purified DR antigens in liposomes stimulate xenogeneic cytolytic T cells in secondary in vitro cultures

Joan C. Gorga, Peter J. Knudsen, Judith A. Foran, Jack L. Strominger, Steven J. Burakoff

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

A monoclonal antibody directed against the human class II major histocompatibility antigen DR was generated. Use of this antibody, LB3.1, allowed isolation of large amounts of highly purified DR by immunoaffinity chromatography. The DR was reconstituted into liposomes and shown to stimulate secondary xenogeneic cytolytic T lymphocytes (CTL) specific for targets expressing DR antigens. DR digested with neuraminidase was equally as effective as native DR at stimulating CTL, while denatured DR and other purified membrane proteins were much less effective. The DR liposome-induced CTL lysed only target cells expressing class II antigens. Cytolysis of targets bearing class II antigens was blocked by DR-specific antisera.

Original languageEnglish
Pages (from-to)160-173
Number of pages14
JournalCellular Immunology
Volume103
Issue number1
DOIs
StatePublished - Nov 1986
Externally publishedYes

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