Two human tissue culture cell lines, LI-1 derived from a patient with acute myelogenous leukemia and IM-1 from a patient with lymphoblastic lymphoma, were compared with donor tissues. Morphologic and functional features were assessed to determine if there were significant differences between the culture lines and the malignant tumor cells of the corresponding patients. The LI-1 line contained mostly large primitive cells which did not resemble the myeloblasts of the donor. The IM-1 cell line was composed of similar primitive cells, lymphocytes, and a few macrophages, whereas the source of this line was a lymphoid tissue composed of a mixture of plasma and lymphoid cells. The two cell lines produce electrophoretically discrete im-munoglobulins in culture. Cell line LI-1 synthesized IgG-Kand IgG-λ. Cell line IM-1 produces electrophoretically discrete IgM-K, IgG-K, and K light polypeptide chains. Electrophoreti-cally discrete immunoglobulins comparable to the imraunoglob-ulins synthesized in culture, however, were not detected in the patients’ serum or synthesized by their tissues. These studies indicate that cell lines established in culture differ significantly from the major portion of malignant cells seen in vivo in these patients. Designation of such cell lines as leukemia cell lines seems inappropriate until further observations can be made on the relationship between the cells in culture and the normal and malignant cells of donor patients. remains unknown, however, whether the original cells in culture synthesized immunoglobulins or whether this capacity developed in the culture environment. The successful establishment of continuous white cell cultures from two patients who are still alive has given us an opportunity to investigate this question. Morphologic, immunochemical, immunofluorescent, and biosynthetic methods were used to compare freshly obtained tissue with the cell lines established and growing in tissue culture.
|Number of pages||12|
|State||Published - Aug 1968|