Immunochemical and functional analysis of Ia-like antigens on human monocyte-macrophages with monoclonal antibodies

Ah Kau Ng; Yu Hui Zhang; Carlo Russo; Soldano Ferrone

doi:10.1016/0008-8749(82)90159-9

Immunochemical and functional analysis of Ia-like antigens on human monocyte-macrophages with monoclonal antibodies

Ah Kau Ng
, Yu Hui Zhang
, Carlo Russo
, Soldano Ferrone

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The distribution, structural profile and functional properties of Ia-like antigens synthesized by human monocyte-macrophages have been analyzed using monoclonal antibodies to common determinants of these antigens. Up to 45 and 70%- of monocyte-macrophages isolated from the fluid of blisters induced with cantharidin and from peripheral blood, respectively, react with monoclonal antibodies to human Ia-like antigens. The level of Ia-like antigens on monocytes-macrophages appears to be similar to that on cultured B lymphoid cells. Monoclonal antibodies to common determinants of Ia-like antigens specifically block antigen presentation by monocyte-macrophages to T lymphocytes as well as proliferative response of T lymphocytes to autologous and allogeneic monocytes-macrophages. These results indicate that common determinants of Ia-like antigens play a role in the interaction of monocytes-macrophages with T lymphocytes.

Original language	English
Pages (from-to)	78-87
Number of pages	10
Journal	Cellular Immunology
Volume	66
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(82)90159-9
State	Published - 1 Jan 1982
Externally published	Yes

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@article{5b50d049dfbc480eb7f019918d38dc4d,

title = "Immunochemical and functional analysis of Ia-like antigens on human monocyte-macrophages with monoclonal antibodies",

abstract = "The distribution, structural profile and functional properties of Ia-like antigens synthesized by human monocyte-macrophages have been analyzed using monoclonal antibodies to common determinants of these antigens. Up to 45 and 70\%- of monocyte-macrophages isolated from the fluid of blisters induced with cantharidin and from peripheral blood, respectively, react with monoclonal antibodies to human Ia-like antigens. The level of Ia-like antigens on monocytes-macrophages appears to be similar to that on cultured B lymphoid cells. Monoclonal antibodies to common determinants of Ia-like antigens specifically block antigen presentation by monocyte-macrophages to T lymphocytes as well as proliferative response of T lymphocytes to autologous and allogeneic monocytes-macrophages. These results indicate that common determinants of Ia-like antigens play a role in the interaction of monocytes-macrophages with T lymphocytes.",

author = "Ng, \{Ah Kau\} and Zhang, \{Yu Hui\} and Carlo Russo and Soldano Ferrone",

note = "Funding Information: This work was supportedb y the National Instituteso f Health Grants 7 ROl AI 19189,7 ROl CA 32609,C A 32634,C A 24329,a nd CA 32635,a nd a Special Fellowship of the Leukemia Society of America (A. K. Ng). Dr. Y.-H. Zhang was a visiting investigatorf rom the Cancer Institute, Chinese Academyo f Medical Sciences,B eijing, People{\textquoteright}sR epublic of China, and was supportedb y an ICRETT grant awardedb y the InternationalU nion Against Cancer, Geneva,S witzerland.T he authorsw ish to acknowledgeth e excellents ecretariala ssistanceo f Ms. Ellen Schmedinga nd Mr. Keith Dunn.",

year = "1982",

month = jan,

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doi = "10.1016/0008-8749(82)90159-9",

language = "English",

volume = "66",

pages = "78--87",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

number = "1",

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T1 - Immunochemical and functional analysis of Ia-like antigens on human monocyte-macrophages with monoclonal antibodies

AU - Ng, Ah Kau

AU - Zhang, Yu Hui

AU - Russo, Carlo

AU - Ferrone, Soldano

N1 - Funding Information: This work was supportedb y the National Instituteso f Health Grants 7 ROl AI 19189,7 ROl CA 32609,C A 32634,C A 24329,a nd CA 32635,a nd a Special Fellowship of the Leukemia Society of America (A. K. Ng). Dr. Y.-H. Zhang was a visiting investigatorf rom the Cancer Institute, Chinese Academyo f Medical Sciences,B eijing, People’sR epublic of China, and was supportedb y an ICRETT grant awardedb y the InternationalU nion Against Cancer, Geneva,S witzerland.T he authorsw ish to acknowledgeth e excellents ecretariala ssistanceo f Ms. Ellen Schmedinga nd Mr. Keith Dunn.

PY - 1982/1/1

Y1 - 1982/1/1

N2 - The distribution, structural profile and functional properties of Ia-like antigens synthesized by human monocyte-macrophages have been analyzed using monoclonal antibodies to common determinants of these antigens. Up to 45 and 70%- of monocyte-macrophages isolated from the fluid of blisters induced with cantharidin and from peripheral blood, respectively, react with monoclonal antibodies to human Ia-like antigens. The level of Ia-like antigens on monocytes-macrophages appears to be similar to that on cultured B lymphoid cells. Monoclonal antibodies to common determinants of Ia-like antigens specifically block antigen presentation by monocyte-macrophages to T lymphocytes as well as proliferative response of T lymphocytes to autologous and allogeneic monocytes-macrophages. These results indicate that common determinants of Ia-like antigens play a role in the interaction of monocytes-macrophages with T lymphocytes.

AB - The distribution, structural profile and functional properties of Ia-like antigens synthesized by human monocyte-macrophages have been analyzed using monoclonal antibodies to common determinants of these antigens. Up to 45 and 70%- of monocyte-macrophages isolated from the fluid of blisters induced with cantharidin and from peripheral blood, respectively, react with monoclonal antibodies to human Ia-like antigens. The level of Ia-like antigens on monocytes-macrophages appears to be similar to that on cultured B lymphoid cells. Monoclonal antibodies to common determinants of Ia-like antigens specifically block antigen presentation by monocyte-macrophages to T lymphocytes as well as proliferative response of T lymphocytes to autologous and allogeneic monocytes-macrophages. These results indicate that common determinants of Ia-like antigens play a role in the interaction of monocytes-macrophages with T lymphocytes.

UR - https://www.scopus.com/pages/publications/0020086490

U2 - 10.1016/0008-8749(82)90159-9

DO - 10.1016/0008-8749(82)90159-9

M3 - Article

C2 - 6177422

AN - SCOPUS:0020086490

SN - 0008-8749

VL - 66

SP - 78

EP - 87

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -

Immunochemical and functional analysis of Ia-like antigens on human monocyte-macrophages with monoclonal antibodies

Abstract

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