Immunochemical analysis of anti-HLA-A2, HLA-A3, and HLA-B27 xenoantisera elicited with hybrids between human and murine cells

Michele A. Pellegrino; Ah Kau Ng; Carlo Russo; Mark Glassy; Soldano Ferrone

doi:10.1007/BF00344307

Immunochemical analysis of anti-HLA-A2, HLA-A3, and HLA-B27 xenoantisera elicited with hybrids between human and murine cells

Michele A. Pellegrino, Ah Kau Ng, Carlo Russo, Mark Glassy, Soldano Ferrone

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8 Scopus citations

Abstract

Rabbits were immunized with hybrids constructed with human and murine cells. Serological and immunochemical studies showed that xenoantiserum 1595 is operationally specific for HLA-A2, xenoantisera 0806 and 0746 for HLA-A3 and xenoantiserum 0745 for HLA-1327. The Fab2 blocking assay suggests a spatial relationship between allotypic determinants recognized by the xenoantisera and those reacting with the HLA-A, B-specific monoclonal antibodies (MoAb) Q1/28,6/3 1, Q6/64, and CR-1 and the anti-β₂-microglobulin (β2-μ) MoAb NAMB-1.

Original language	English
Pages (from-to)	141-152
Number of pages	12
Journal	Immunogenetics
Volume	14
Issue number	1-2
DOIs	https://doi.org/10.1007/BF00344307
State	Published - Sep 1981
Externally published	Yes

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title = "Immunochemical analysis of anti-HLA-A2, HLA-A3, and HLA-B27 xenoantisera elicited with hybrids between human and murine cells",

abstract = "Rabbits were immunized with hybrids constructed with human and murine cells. Serological and immunochemical studies showed that xenoantiserum 1595 is operationally specific for HLA-A2, xenoantisera 0806 and 0746 for HLA-A3 and xenoantiserum 0745 for HLA-1327. The Fab2 blocking assay suggests a spatial relationship between allotypic determinants recognized by the xenoantisera and those reacting with the HLA-A, B-specific monoclonal antibodies (MoAb) Q1/28,6/3 1, Q6/64, and CR-1 and the anti-β2-microglobulin (β2-μ) MoAb NAMB-1.",

author = "Pellegrino, {Michele A.} and Ng, {Ah Kau} and Carlo Russo and Mark Glassy and Soldano Ferrone",

year = "1981",

month = sep,

doi = "10.1007/BF00344307",

language = "English",

volume = "14",

pages = "141--152",

journal = "Immunogenetics",

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T1 - Immunochemical analysis of anti-HLA-A2, HLA-A3, and HLA-B27 xenoantisera elicited with hybrids between human and murine cells

AU - Pellegrino, Michele A.

AU - Ng, Ah Kau

AU - Russo, Carlo

AU - Glassy, Mark

AU - Ferrone, Soldano

PY - 1981/9

Y1 - 1981/9

N2 - Rabbits were immunized with hybrids constructed with human and murine cells. Serological and immunochemical studies showed that xenoantiserum 1595 is operationally specific for HLA-A2, xenoantisera 0806 and 0746 for HLA-A3 and xenoantiserum 0745 for HLA-1327. The Fab2 blocking assay suggests a spatial relationship between allotypic determinants recognized by the xenoantisera and those reacting with the HLA-A, B-specific monoclonal antibodies (MoAb) Q1/28,6/3 1, Q6/64, and CR-1 and the anti-β2-microglobulin (β2-μ) MoAb NAMB-1.

AB - Rabbits were immunized with hybrids constructed with human and murine cells. Serological and immunochemical studies showed that xenoantiserum 1595 is operationally specific for HLA-A2, xenoantisera 0806 and 0746 for HLA-A3 and xenoantiserum 0745 for HLA-1327. The Fab2 blocking assay suggests a spatial relationship between allotypic determinants recognized by the xenoantisera and those reacting with the HLA-A, B-specific monoclonal antibodies (MoAb) Q1/28,6/3 1, Q6/64, and CR-1 and the anti-β2-microglobulin (β2-μ) MoAb NAMB-1.

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SN - 0093-7711

VL - 14

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JO - Immunogenetics

JF - Immunogenetics

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ER -

Immunochemical analysis of anti-HLA-A2, HLA-A3, and HLA-B27 xenoantisera elicited with hybrids between human and murine cells

Abstract

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