Immunization with a synthetic multiepitope antigen induces humoral and cellular immune responses to hepatitis C virus in mice

Lishan Chen; Tienjun Gao; Nan Yang; Jiansheng Huang; Yuhan Chen; Tong Gao; Qihan Li; Daming Ren

doi:10.1089/vim.2006.0067

Immunization with a synthetic multiepitope antigen induces humoral and cellular immune responses to hepatitis C virus in mice

Lishan Chen, Tienjun Gao, Nan Yang, Jiansheng Huang, Yuhan Chen, Tong Gao, Qihan Li, Daming Ren

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The immunogenicity of a synthetic multiepitope PCX3 antigen, which contains triple tandem repeats of five conserved epitopes from hepatitis C virus (HCV) polyprotein, was studied in BALB/c mice given three intraperitoneal injections of antigen with Freund's adjuvant. Both a strong antibody response and specific cytotoxic T lymphocytes were induced. The specific anti-PCX3 IgG was able to bind HCV particles from hepatitis C patient sera by incubation overnight. In particular, in transgenic mice with chimeric human livers, anti-PCX3 antibody was able to lower the viral load in two of five mice and to eliminate HCV infection in three of five mice by 2 wk after inoculation with HCV-positive serum from patients. These results indicated that the synthetic multiepitope PCX3 antigen elicits a potent humoral and cellular immune response against HCV.

Original language	English
Pages (from-to)	170-179
Number of pages	10
Journal	Viral Immunology
Volume	20
Issue number	1
DOIs	https://doi.org/10.1089/vim.2006.0067
State	Published - 2007
Externally published	Yes

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title = "Immunization with a synthetic multiepitope antigen induces humoral and cellular immune responses to hepatitis C virus in mice",

abstract = "The immunogenicity of a synthetic multiepitope PCX3 antigen, which contains triple tandem repeats of five conserved epitopes from hepatitis C virus (HCV) polyprotein, was studied in BALB/c mice given three intraperitoneal injections of antigen with Freund's adjuvant. Both a strong antibody response and specific cytotoxic T lymphocytes were induced. The specific anti-PCX3 IgG was able to bind HCV particles from hepatitis C patient sera by incubation overnight. In particular, in transgenic mice with chimeric human livers, anti-PCX3 antibody was able to lower the viral load in two of five mice and to eliminate HCV infection in three of five mice by 2 wk after inoculation with HCV-positive serum from patients. These results indicated that the synthetic multiepitope PCX3 antigen elicits a potent humoral and cellular immune response against HCV.",

author = "Lishan Chen and Tienjun Gao and Nan Yang and Jiansheng Huang and Yuhan Chen and Tong Gao and Qihan Li and Daming Ren",

year = "2007",

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T1 - Immunization with a synthetic multiepitope antigen induces humoral and cellular immune responses to hepatitis C virus in mice

AU - Chen, Lishan

AU - Gao, Tienjun

AU - Yang, Nan

AU - Huang, Jiansheng

AU - Chen, Yuhan

AU - Gao, Tong

AU - Li, Qihan

AU - Ren, Daming

PY - 2007

Y1 - 2007

N2 - The immunogenicity of a synthetic multiepitope PCX3 antigen, which contains triple tandem repeats of five conserved epitopes from hepatitis C virus (HCV) polyprotein, was studied in BALB/c mice given three intraperitoneal injections of antigen with Freund's adjuvant. Both a strong antibody response and specific cytotoxic T lymphocytes were induced. The specific anti-PCX3 IgG was able to bind HCV particles from hepatitis C patient sera by incubation overnight. In particular, in transgenic mice with chimeric human livers, anti-PCX3 antibody was able to lower the viral load in two of five mice and to eliminate HCV infection in three of five mice by 2 wk after inoculation with HCV-positive serum from patients. These results indicated that the synthetic multiepitope PCX3 antigen elicits a potent humoral and cellular immune response against HCV.

AB - The immunogenicity of a synthetic multiepitope PCX3 antigen, which contains triple tandem repeats of five conserved epitopes from hepatitis C virus (HCV) polyprotein, was studied in BALB/c mice given three intraperitoneal injections of antigen with Freund's adjuvant. Both a strong antibody response and specific cytotoxic T lymphocytes were induced. The specific anti-PCX3 IgG was able to bind HCV particles from hepatitis C patient sera by incubation overnight. In particular, in transgenic mice with chimeric human livers, anti-PCX3 antibody was able to lower the viral load in two of five mice and to eliminate HCV infection in three of five mice by 2 wk after inoculation with HCV-positive serum from patients. These results indicated that the synthetic multiepitope PCX3 antigen elicits a potent humoral and cellular immune response against HCV.

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DO - 10.1089/vim.2006.0067

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AN - SCOPUS:34247191554

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JO - Viral Immunology

JF - Viral Immunology

IS - 1

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Immunization with a synthetic multiepitope antigen induces humoral and cellular immune responses to hepatitis C virus in mice

Abstract

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