TY - JOUR
T1 - Immune transcriptome alterations in the temporal cortex of subjects with autism
AU - Garbett, Krassimira
AU - Ebert, Philip J.
AU - Mitchell, Amanda
AU - Lintas, Carla
AU - Manzi, Barbara
AU - Mirnics, Károly
AU - Persico, Antonio M.
N1 - Funding Information:
We would like to thank Dr. Dominique Arion for her assistance in tissue processing, Dr. Pat Levitt for the critical input on project design and Dr. Luca Battistini for the helpful comments on the manuscript. We also want to thank the donor families, the Autism Tissue Program (Princeton, N.J.) and the ATP Director, Dr. Jane Pickett, for providing the tissue resources for this study. The current research was supported by K02 MH070786 and R01 MH079299 (KM), a NARSAD Young Investigator fellowship (PE) and MIUR PRIN 200605819 (AP). Appendix A
PY - 2008/6
Y1 - 2008/6
N2 - Autism is a severe disorder that involves both genetic and environmental factors. Expression profiling of the superior temporal gyrus of six autistic subjects and matched controls revealed increased transcript levels of many immune system-related genes. We also noticed changes in transcripts related to cell communication, differentiation, cell cycle regulation and chaperone systems. Critical expression changes were confirmed by qPCR (BCL6, CHI3L1, CYR61, IFI16, IFITM3, MAP2K3, PTDSR, RFX4, SPP1, RELN, NOTCH2, RIT1, SFN, GADD45B, HSPA6, HSPB8 and SERPINH1). Overall, these expression patterns appear to be more associated with the late recovery phase of autoimmune brain disorders, than with the innate immune response characteristic of neurodegenerative diseases. Moreover, a variance-based analysis revealed much greater transcript variability in brains from autistic subjects compared to the control group, suggesting that these genes may represent autism susceptibility genes and should be assessed in follow-up genetic studies.
AB - Autism is a severe disorder that involves both genetic and environmental factors. Expression profiling of the superior temporal gyrus of six autistic subjects and matched controls revealed increased transcript levels of many immune system-related genes. We also noticed changes in transcripts related to cell communication, differentiation, cell cycle regulation and chaperone systems. Critical expression changes were confirmed by qPCR (BCL6, CHI3L1, CYR61, IFI16, IFITM3, MAP2K3, PTDSR, RFX4, SPP1, RELN, NOTCH2, RIT1, SFN, GADD45B, HSPA6, HSPB8 and SERPINH1). Overall, these expression patterns appear to be more associated with the late recovery phase of autoimmune brain disorders, than with the innate immune response characteristic of neurodegenerative diseases. Moreover, a variance-based analysis revealed much greater transcript variability in brains from autistic subjects compared to the control group, suggesting that these genes may represent autism susceptibility genes and should be assessed in follow-up genetic studies.
KW - Autism
KW - DNA microarray
KW - Gene expression
KW - Post mortem
KW - Temporal cortex
KW - Transcriptome
KW - qPCR
UR - http://www.scopus.com/inward/record.url?scp=43849112803&partnerID=8YFLogxK
U2 - 10.1016/j.nbd.2008.01.012
DO - 10.1016/j.nbd.2008.01.012
M3 - Article
C2 - 18378158
AN - SCOPUS:43849112803
SN - 0969-9961
VL - 30
SP - 303
EP - 311
JO - Neurobiology of Disease
JF - Neurobiology of Disease
IS - 3
ER -