TY - JOUR
T1 - Immune phenotype of peripheral blood mononuclear cells in patients with high-risk non-muscle invasive bladder cancer
AU - Audenet, François
AU - Farkas, Adam M.
AU - Anastos, Harry
AU - Galsky, Matthew D.
AU - Bhardwaj, Nina
AU - Sfakianos, John P.
N1 - Funding Information:
Conflict of interest NB is supported by the Parker Institute for Cancer Immunotherapy. AMF is supported by an NIH Immunology Training Grant (T32AI007605).
Funding Information:
Author contributions FA: project development, data collection, data analysis, manuscript writing. AMF: project development, data collection, data analysis, manuscript editing. HA: data collection, manuscript editing. MDG: project development, data analysis, manuscript editing. NB: project development, data analysis, manuscript editing. JPS: project development, data collection, data analysis, manuscript editing Funding These studies were funded by grants from the Cancer Research Institute and from the National Institutes of Health (R01 CA201189).
Publisher Copyright:
© Springer-Verlag GmbH Germany, part of Springer Nature 2018.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Purpose To explore the immune phenotype of peripheral blood mononuclear cells (PBMC) in patients with high-risk nonmuscle invasive bladder cancer (NMIBC). Methods We prospectively collected blood samples from patients with high-risk NMIBC treated at our institution. PBMC were analyzed by flow cytometry to determine the frequency of T cells and NK cells and the expression of immunoregulatory molecules (Tim-3, TIGIT and PD-1). PBMC from healthy donors (HD) were included for comparison, and associations with response to BCG were investigated. Results A total of 38 patients were included, 19 BCG responders and 19 BCG refractory. Compared to 16 PBMC from HD, the frequency of total NK cells was significantly higher in patients with NMIBC [15.2% (IQR: 11.4, 22.2) vs. 5.72% (IQR: 4.84, 9.79); p = 0.05], whereas the frequency of T cells was not statistically different. Both Tim-3 and TIGIT expressions were significantly higher in NMIBC compared to HD, particularly in NK cells [13.8% (11.0; 22.4) vs. 5.56% (4.20; 10.2) and 34.9% (18.9; 53.5) vs. 1.82% (0.63; 5.16), respectively; p < 0.001]. Overall, the expression of PD-1 in all cell types was low in both NMIBC patients and HD. The immune phenotype was not significantly different before and after initiation of BCG. However, the proportion of CD8 + T cells before BCG was significantly higher in responders. Conclusion The immune phenotype of PBMC from patients with high-risk NMIBC was significantly different from HD, regardless of the presence of disease or the initiation of BCG. Peripheral CD8 + T cells could play a role in response to BCG.
AB - Purpose To explore the immune phenotype of peripheral blood mononuclear cells (PBMC) in patients with high-risk nonmuscle invasive bladder cancer (NMIBC). Methods We prospectively collected blood samples from patients with high-risk NMIBC treated at our institution. PBMC were analyzed by flow cytometry to determine the frequency of T cells and NK cells and the expression of immunoregulatory molecules (Tim-3, TIGIT and PD-1). PBMC from healthy donors (HD) were included for comparison, and associations with response to BCG were investigated. Results A total of 38 patients were included, 19 BCG responders and 19 BCG refractory. Compared to 16 PBMC from HD, the frequency of total NK cells was significantly higher in patients with NMIBC [15.2% (IQR: 11.4, 22.2) vs. 5.72% (IQR: 4.84, 9.79); p = 0.05], whereas the frequency of T cells was not statistically different. Both Tim-3 and TIGIT expressions were significantly higher in NMIBC compared to HD, particularly in NK cells [13.8% (11.0; 22.4) vs. 5.56% (4.20; 10.2) and 34.9% (18.9; 53.5) vs. 1.82% (0.63; 5.16), respectively; p < 0.001]. Overall, the expression of PD-1 in all cell types was low in both NMIBC patients and HD. The immune phenotype was not significantly different before and after initiation of BCG. However, the proportion of CD8 + T cells before BCG was significantly higher in responders. Conclusion The immune phenotype of PBMC from patients with high-risk NMIBC was significantly different from HD, regardless of the presence of disease or the initiation of BCG. Peripheral CD8 + T cells could play a role in response to BCG.
KW - BCG
KW - Immune checkpoints
KW - Immune phenotype
KW - Non-muscle invasive bladder cancer
KW - Peripheral blood mononuclear cells
KW - Predictive factors
UR - http://www.scopus.com/inward/record.url?scp=85047940057&partnerID=8YFLogxK
U2 - 10.1007/s00345-018-2359-7
DO - 10.1007/s00345-018-2359-7
M3 - Article
C2 - 29860605
AN - SCOPUS:85047940057
SN - 0724-4983
VL - 36
SP - 1741
EP - 1748
JO - World Journal of Urology
JF - World Journal of Urology
IS - 11
ER -