Immature mDA neurons ameliorate motor deficits in a 6-OHDA Parkinson's disease mouse model and are functional after cryopreservation

Dominique Leitner, Mahesh Ramamoorthy, Marion Dejosez, Thomas P. Zwaka

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Parkinson's disease is associated with the loss of dopaminergic neurons in the midbrain. Clinical studies investigating replacement of these neurons with in vitro-generated neurons are currently underway. However, this approach has been limited by difficulties in scaling up on-demand production of midbrain dopaminergic (mDA) neurons from pluripotent stem cells. Cryo-preservation may offer a solution, as it allows for banking of quality controlled mDA neurons. In this study, we tested different freezing conditions and found that optimal cryopreservation of immature human mDA neurons at an early differentiation time point was achieved in STEM-CELLBANKER medium using a controlled freezing program.

Original languageEnglish
Article number101617
JournalStem Cell Research
Volume41
DOIs
StatePublished - Dec 2019

Keywords

  • 6-OHDA mouse model
  • Cryopreservation
  • Human induced pluripotent stem cells
  • Midbrain dopaminergic neurons
  • Parkinson's disease

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