Imaging with radiolabeled antisense oligonucleotides for the detection of intracellular messenger RNA and cardiovascular disease

M. K. Dewanjee; N. Haider; J. Narula

doi:10.1016/S1071-3581(99)90047-8

Imaging with radiolabeled antisense oligonucleotides for the detection of intracellular messenger RNA and cardiovascular disease

M. K. Dewanjee, N. Haider, J. Narula

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Original language	English
Pages (from-to)	345-356
Number of pages	12
Journal	Journal of Nuclear Cardiology
Volume	6
Issue number	3
DOIs	https://doi.org/10.1016/S1071-3581(99)90047-8
State	Published - 1999
Externally published	Yes

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10.1016/S1071-3581(99)90047-8

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@article{0fb3ee98537447c5b4c304118354e6b2,

title = "Imaging with radiolabeled antisense oligonucleotides for the detection of intracellular messenger RNA and cardiovascular disease",

author = "Dewanjee, {M. K.} and N. Haider and J. Narula",

note = "Funding Information: If the antisense probe is appropriately radiolabeled, it should allow a noninvasive diagnostic strategy for evaluation of mRNA associated with the disease process. For the development of effective imaging strategy, it is necessary that there is sufficient increase in mRNA production, that the antisense oligonucleotide (ASON) probe is appropriately radiolabeled at high specific activity and is able to negotiate through the cell membrane into the cytoplasm. The ASON probe should be stable against degradation from nucleases and should comprise high enough specific activity to detect relatively low levels of From the Departmento f VeterinaryB iosciences,U niversityo f Illinois, Urbana, Illinois, and aAllegheny University Hospital, Hahnemann Division, Philadelphia, Pennsylvania. Developmento f radiolabeledp robes and their applications indiagnostic imaging were funded by the University of Illinois Research Board (UI-1-2-69410 Reprint requests: Mrinal K. Dewanjee,P hD, Department of Veterinary Biosciences, 3215 VMBS Building, Universityo f Illinois at Urbana-Champaign, 2001 S Lincoln Ave, Urbana, IL 61802; [email protected]. J Nucl Cardiol 1999;6:345-56. Copyright {\textcopyright} 1999 by the American Society of Nuclear Cardiology. 1071-3581/99/$8.00 + 0 43/1/98694 mRNA. Regardless of whether the gene of interest is an endogenous gene or exogenously administered (gene therapy), the same type of probes are used for the imaging of gene expression. The radiolabeled probes administered at microgram level may not affect transcription.",

year = "1999",

doi = "10.1016/S1071-3581(99)90047-8",

language = "English",

volume = "6",

pages = "345--356",

journal = "Journal of Nuclear Cardiology",

issn = "1071-3581",

publisher = "American Society of Nuclear Cardiology",

number = "3",

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TY - JOUR

T1 - Imaging with radiolabeled antisense oligonucleotides for the detection of intracellular messenger RNA and cardiovascular disease

AU - Dewanjee, M. K.

AU - Haider, N.

AU - Narula, J.

N1 - Funding Information: If the antisense probe is appropriately radiolabeled, it should allow a noninvasive diagnostic strategy for evaluation of mRNA associated with the disease process. For the development of effective imaging strategy, it is necessary that there is sufficient increase in mRNA production, that the antisense oligonucleotide (ASON) probe is appropriately radiolabeled at high specific activity and is able to negotiate through the cell membrane into the cytoplasm. The ASON probe should be stable against degradation from nucleases and should comprise high enough specific activity to detect relatively low levels of From the Departmento f VeterinaryB iosciences,U niversityo f Illinois, Urbana, Illinois, and aAllegheny University Hospital, Hahnemann Division, Philadelphia, Pennsylvania. Developmento f radiolabeledp robes and their applications indiagnostic imaging were funded by the University of Illinois Research Board (UI-1-2-69410 Reprint requests: Mrinal K. Dewanjee,P hD, Department of Veterinary Biosciences, 3215 VMBS Building, Universityo f Illinois at Urbana-Champaign, 2001 S Lincoln Ave, Urbana, IL 61802; [email protected]. J Nucl Cardiol 1999;6:345-56. Copyright © 1999 by the American Society of Nuclear Cardiology. 1071-3581/99/$8.00 + 0 43/1/98694 mRNA. Regardless of whether the gene of interest is an endogenous gene or exogenously administered (gene therapy), the same type of probes are used for the imaging of gene expression. The radiolabeled probes administered at microgram level may not affect transcription.

PY - 1999

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