IL-7 induces proliferation, variable cytokine-producing ability and IL- 2 responsiveness in naive CD4+ T-cells from human cord blood

Tetsuya Fukui; Kenji Katamura; Nobutaka Abe; Takahiro Kiyomasu; Jun Iio; Hideki Ueno; Mitsufumi Mayumi; Kenshi Furusho

doi:10.1016/S0165-2478(97)00093-X

IL-7 induces proliferation, variable cytokine-producing ability and IL- 2 responsiveness in naive CD4⁺ T-cells from human cord blood

Tetsuya Fukui, Kenji Katamura, Nobutaka Abe, Takahiro Kiyomasu, Jun Iio, Hideki Ueno, Mitsufumi Mayumi, Kenshi Furusho

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

We investigated the effects of IL-7 on the proliferation and acquisition of cytokine-producing ability of naive CD4⁺ T-cells from human cord blood. Naive CD4⁺ CD45RA⁺ T-cells from human cord blood expressed CDw127 (IL-7R) at higher levels than adult CD4⁺ CD45RA⁺ T-cell and produced IL-2 and a small amount of IFN-γ upon stimulation with PMA and ionomycin. IL-7 induced IL-2-independent proliferation and both Th1- and Th2-type cytokine-producing abilities in cord blood CD4⁺ CD45RA⁺ T-cells without stimulation via TCR. These results suggest that this IL-7-induced antigen-independent activation mechanism could contribute to maintaining the clonal size of naive T-cells with the potential to differentiate into either Th1 or Th2 cells at the sites of IL-7-expression.

Original language	English
Pages (from-to)	21-28
Number of pages	8
Journal	Immunology Letters
Volume	59
Issue number	1
DOIs	https://doi.org/10.1016/S0165-2478(97)00093-X
State	Published - Oct 1997
Externally published	Yes

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@article{6a3bd80176f94303aa54118a1cb10e85,

title = "IL-7 induces proliferation, variable cytokine-producing ability and IL- 2 responsiveness in naive CD4+ T-cells from human cord blood",

abstract = "We investigated the effects of IL-7 on the proliferation and acquisition of cytokine-producing ability of naive CD4+ T-cells from human cord blood. Naive CD4+ CD45RA+ T-cells from human cord blood expressed CDw127 (IL-7R) at higher levels than adult CD4+ CD45RA+ T-cell and produced IL-2 and a small amount of IFN-γ upon stimulation with PMA and ionomycin. IL-7 induced IL-2-independent proliferation and both Th1- and Th2-type cytokine-producing abilities in cord blood CD4+ CD45RA+ T-cells without stimulation via TCR. These results suggest that this IL-7-induced antigen-independent activation mechanism could contribute to maintaining the clonal size of naive T-cells with the potential to differentiate into either Th1 or Th2 cells at the sites of IL-7-expression.",

author = "Tetsuya Fukui and Kenji Katamura and Nobutaka Abe and Takahiro Kiyomasu and Jun Iio and Hideki Ueno and Mitsufumi Mayumi and Kenshi Furusho",

note = "Funding Information: We are indebted to Dr Inoue and the nurses at the Obstetrics Department of Kyoto University Hospital and Dr Narumoto in Ishida Hospital for kindly collecting cord blood samples. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan and grants from the Pollution-related Health Damage Compensation and Prevention Association and The Mother and Child Health Foundation.",

year = "1997",

month = oct,

doi = "10.1016/S0165-2478(97)00093-X",

language = "English",

volume = "59",

pages = "21--28",

journal = "Immunology Letters",

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T1 - IL-7 induces proliferation, variable cytokine-producing ability and IL- 2 responsiveness in naive CD4+ T-cells from human cord blood

AU - Fukui, Tetsuya

AU - Katamura, Kenji

AU - Abe, Nobutaka

AU - Kiyomasu, Takahiro

AU - Iio, Jun

AU - Ueno, Hideki

AU - Mayumi, Mitsufumi

AU - Furusho, Kenshi

N1 - Funding Information: We are indebted to Dr Inoue and the nurses at the Obstetrics Department of Kyoto University Hospital and Dr Narumoto in Ishida Hospital for kindly collecting cord blood samples. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan and grants from the Pollution-related Health Damage Compensation and Prevention Association and The Mother and Child Health Foundation.

PY - 1997/10

Y1 - 1997/10

N2 - We investigated the effects of IL-7 on the proliferation and acquisition of cytokine-producing ability of naive CD4+ T-cells from human cord blood. Naive CD4+ CD45RA+ T-cells from human cord blood expressed CDw127 (IL-7R) at higher levels than adult CD4+ CD45RA+ T-cell and produced IL-2 and a small amount of IFN-γ upon stimulation with PMA and ionomycin. IL-7 induced IL-2-independent proliferation and both Th1- and Th2-type cytokine-producing abilities in cord blood CD4+ CD45RA+ T-cells without stimulation via TCR. These results suggest that this IL-7-induced antigen-independent activation mechanism could contribute to maintaining the clonal size of naive T-cells with the potential to differentiate into either Th1 or Th2 cells at the sites of IL-7-expression.

AB - We investigated the effects of IL-7 on the proliferation and acquisition of cytokine-producing ability of naive CD4+ T-cells from human cord blood. Naive CD4+ CD45RA+ T-cells from human cord blood expressed CDw127 (IL-7R) at higher levels than adult CD4+ CD45RA+ T-cell and produced IL-2 and a small amount of IFN-γ upon stimulation with PMA and ionomycin. IL-7 induced IL-2-independent proliferation and both Th1- and Th2-type cytokine-producing abilities in cord blood CD4+ CD45RA+ T-cells without stimulation via TCR. These results suggest that this IL-7-induced antigen-independent activation mechanism could contribute to maintaining the clonal size of naive T-cells with the potential to differentiate into either Th1 or Th2 cells at the sites of IL-7-expression.

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AN - SCOPUS:0030984465

SN - 0165-2478

VL - 59

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EP - 28

JO - Immunology Letters

JF - Immunology Letters

IS - 1

ER -

IL-7 induces proliferation, variable cytokine-producing ability and IL- 2 responsiveness in naive CD4+ T-cells from human cord blood

Abstract

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IL-7 induces proliferation, variable cytokine-producing ability and IL- 2 responsiveness in naive CD4⁺ T-cells from human cord blood