IL-2 up-regulates but IFN-γ suppresses IL-8 expression in human monocytes

G. L. Gusella, T. Musso, M. C. Bosco, I. Espinoza-Delgado, K. Matsushima, L. Varesio

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86 Scopus citations

Abstract

IL-2 has pleiotropic properties and is a potent activator of monocytic functions. Since monocytes are an important source of the chemoattractant cytokine IL-8, we studied the effects of IL-2 on the expression of IL-8 in human monocytes. IL-8 mRNA expression was detectable in resting human monocytes. Treatment of monocytes with IL-2 increased IL-8 mRNA expression by a protein synthesis-independent process. The augmentation of IL-8 mRNA by IL- 2 was associated with an increase in IL-8 secretion. The expression of IL-8 mRNA was not a nonspecific response to any stimulus of monocyte activation. In fact, IFN-γ, which is also a potent monocyte activator, not only failed to induce IL-8 expression but inhibited the stimulation of IL-8 by IL-2. Nuclear run-on experiments demonstrated that both the enhancement of IL-8 mRNA expression and its down-regulation by IFN-γ occurred at the transcriptional level. These results show for the first time that in fresh human monocytes, IL-8 expression is differentially regulated by IL-2 and IFN- γ and suggest that the interactions among IL-2, IL-8, and IFN-γ may be important for the development and control of the inflammatory response.

Original languageEnglish
Pages (from-to)2725-2732
Number of pages8
JournalJournal of Immunology
Volume151
Issue number5
StatePublished - 1993
Externally publishedYes

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