IL-17 induces radiation resistance of B lymphoma cells by suppressing p53 expression and thereby inhibiting irradiation-triggered apoptosis

Qingshan Li, Xin Xu, Weijie Zhong, Qinghua Du, Bizhen Yu, Huabao Xiong

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

p53 is a well-known tumor suppressor. However, the regulatory mechanism(s) for p53 expression in B lymphoma cells, and the possible role of p53 in the development of the radioresistance in tumor cells are largely unknown. A human B lymphoma cell line, Karpas1106 (k1106), was used as a model of radioresistance. Apoptosis of k1106 cells was determined using flow cytometry. Expression of p53 was assessed using real time RT-PCR and western blotting. The results showed that irradiation at 8 Gy induced apoptosis in up to 40% of k1106 cells. At the same time, the irradiation markedly increased IL-6 production of the k1106 cells. When k1106 cells were cocultured with regulatory T cells (Tregs) and irradiated, the rate of apoptotic k1106 cells was significantly reduced, indicating an acquired resistance to irradiation. IL-6 derived from the irradiation-treated k1106 cells induced IL-17 expression in Tregs. The IL-17 + Foxp3 + T cells suppressed p53 expression in k1106 cells. Collectively, irradiated k1106 cells induce the expression of IL-17 in Tregs, which interferes with the expression of p53 protein in k1106 cells and thereby represses irradiation-triggered apoptosis in k1106 cells.

Original languageEnglish
Pages (from-to)366-372
Number of pages7
JournalCellular and Molecular Immunology
Volume12
Issue number3
DOIs
StatePublished - 7 May 2015

Keywords

  • interleukin-17
  • irradiation
  • lymphoma
  • p53 protein
  • regulatory T cells

Fingerprint

Dive into the research topics of 'IL-17 induces radiation resistance of B lymphoma cells by suppressing p53 expression and thereby inhibiting irradiation-triggered apoptosis'. Together they form a unique fingerprint.

Cite this