Identification of species specific marker(s) in ruminants using mini-/micro-satellite associated sequence amplification (MASA)

Deepak Sharma, A. K. Sharma, S. M. Deb, R. K. Singh, G. S. Bisht

Research output: Contribution to journalArticlepeer-review

Abstract

Oligonucleotides primers (3) commonly used for classical DNA fingerprinting were utilized as single primer to generate mini-/micro-satellite associated sequence amplification (MASA) profiles among the four ruminants (cattle, buffalo, sheep and goat) using step down (SD)-PCR. The primers (O33.15, O33.6 and OAT 18.2) generated total of 41 amplicons on PAGE, out of which 36 were polymorphic. The average number of bands ranged from 4.00 to 13.00 on PAGE with size range of 285 to 2290 bp. Microsatellite primer (OAT 18.2) generated higher number of bands than the minisatellite (O33.15, O33.6) primers. All the three primers produced several species-specific bands. Between species similarity estimates from the O33.6 primer was observed to be maximum for sheep vs. goat (0.696) and cattle vs. buffalo (0.667). Genetic relatedness estimates from the microsatellite (OAT 18.2) primer revealed a close relationship of buffalo with sheep and goat rather than the cattle. In the present study, the application of SD-PCR for MASA in ruminants is being reported for the first time.

Original languageEnglish
Pages (from-to)248-251
Number of pages4
JournalIndian Journal of Animal Sciences
Volume76
Issue number3
StatePublished - Mar 2006
Externally publishedYes

Keywords

  • MASA
  • Microsatellite
  • Minisatellite
  • Ruminants
  • Step down PCR

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