Identification of Somatic Mitochondrial DNA Mutations, Heteroplasmy, and Increased Levels of Catenanes in Tumor Specimens Obtained from Three Endometrial Cancer Patients

Matthew J. Young, Ravi Sachidanandam, Dale B. Hales, Laurent Brard, Kathy Robinson, Md Mostafijur Rahman, Pabitra Khadka, Kathleen Groesch, Carolyn K.J. Young

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Endometrial carcinoma (EC) is the most common type of gynecologic malignant epithelial tumor, with the death rate from this disease doubling over the past 20 years. Mitochondria provide cancer cells with necessary anabolic building blocks such as amino acids, lipids, and nucleotides, and EC samples have been shown to increase mitochondrial biogenesis. In cancer, mitochondrial DNA (mtDNA) heteroplasmy studies suggest that heteroplasmic variants encode predicted pathogenic proteins. We investigated the mtDNA genotypes within peri-normal and tumor specimens obtained from three individuals diagnosed with EC. DNA extracts from peri-normal and tumor tissues were used for mtDNA-specific next-generation sequencing and analyses of mtDNA content and topoisomers. The three tumors harbor heteroplasmic somatic mutations, and at least one mutation in each carcinoma is predicted to deleteriously alter a mtDNA-encoded protein. Somatic heteroplasmy linked to two mtDNA tRNA genes was found in separate tumors, and two heteroplasmic non-coding variants were identified in a single EC tumor. While two tumors had altered mtDNA content, all three displayed increased mtDNA catenanes. Our findings support that EC cells require wild-type mtDNA, but heteroplasmic mutations may alter mitochondrial metabolism to help promote cancer cell growth and proliferation.

Original languageEnglish
Article number562
JournalLife
Volume12
Issue number4
DOIs
StatePublished - Apr 2022

Keywords

  • endometrial cancer
  • heteroplasmy
  • homoplasmy
  • human mitochondrial DNA (mtDNA) maintenance
  • mtDNA content
  • mtDNA copy number
  • mtDNA topoisomers
  • next-generation sequencing (NGS)
  • somatic mutations
  • uterine cancer

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