Background: Caseins are the major allergens responsible for cow's milk allergy (CMA). We have previously identified the IgE-binding epitopes of the major cow's milk (CM) proteins except for αs2-casein. Methods: Overlapping decapeptides representing the entire length of αs2-casein were synthesized on a cellulose-derivatized membrane. Sera from 13 CM-allergic children, 4-15 years of age, with a median level of CM-specific IgE > 100 kU/l (range 33.7 to > 100kU/l) were used to identify IgE-binding epitopes. Results: Four major and six minor sequential IgE-binding regions were identified on αs2-casein. The first major region is located in the middle of the protein at amino acids (AA) 83-100, and the other three major regions are located in the carboxy terminal portion of the protein at AA 143-158, 157-172 and 165-188. The minor IgE-binding regions were identified at AA 31-44, 43-56, 93-106, 105-114, 117-128, and 191-200. Conclusion: We identified 10 sequential IgE-binding regions on αs2-casein and performed the first crucial step in the development of immunotherapeutic interventions for CMA.

Original languageEnglish
Pages (from-to)93-96
Number of pages4
JournalInternational Archives of Allergy and Immunology
Issue number1
StatePublished - 2002


  • Cow's milk allergy
  • Epitope
  • SPOTs membrane
  • Sequential, conformational
  • α-Casein


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