TY - JOUR
T1 - Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI
AU - Ammayappan, Arun
AU - Upadhyay, Chitra
AU - Gelb, Jack
AU - Vakharia, Vikram N.
N1 - Funding Information:
This project was supported by the National Research Initiative of the United States Department of Agriculture (USDA) Cooperative State Research, Education and Extension Service; Grant No. 2004-35204-14814.
PY - 2009/3
Y1 - 2009/3
N2 - Infectious bronchitis virus (IBV) is the causal agent of infectious bronchitis, which still remains one of the most important poultry diseases worldwide because of numerous serotypes and variants. A virulent strain of IBV, isolated from Arkansas (Ark), was propagated in embryonated eggs (Ark DPI 11). Following 101 serial passages in embryonated eggs, an attenuated strain of IBV was established (Ark DPI 101) that does not induce histopathological lesions in the tracheae of infected chicks. To identify sequence changes responsible for the attenuation of IBV, complete genome sequences of both virulent and attenuated Ark DPI viruses were obtained. Comparison of the genome sequences of the virulent and attenuated Ark DPI viruses reveals that these viruses are similar and differ only by 21 nucleotides, resulting in 17 amino acids changes. Most of those substitutions are located in the replicase 1a and spike genes. No differences were observed in gene 3, M or 5a, and only one nucleotide substitution each was present in 5b, N and 3′UTR. By comparing the deduced amino acid sequences of virulent and attenuated viruses, we identified sequence changes responsible for the adaptation and attenuation of the IBV-Ark DPI strain.
AB - Infectious bronchitis virus (IBV) is the causal agent of infectious bronchitis, which still remains one of the most important poultry diseases worldwide because of numerous serotypes and variants. A virulent strain of IBV, isolated from Arkansas (Ark), was propagated in embryonated eggs (Ark DPI 11). Following 101 serial passages in embryonated eggs, an attenuated strain of IBV was established (Ark DPI 101) that does not induce histopathological lesions in the tracheae of infected chicks. To identify sequence changes responsible for the attenuation of IBV, complete genome sequences of both virulent and attenuated Ark DPI viruses were obtained. Comparison of the genome sequences of the virulent and attenuated Ark DPI viruses reveals that these viruses are similar and differ only by 21 nucleotides, resulting in 17 amino acids changes. Most of those substitutions are located in the replicase 1a and spike genes. No differences were observed in gene 3, M or 5a, and only one nucleotide substitution each was present in 5b, N and 3′UTR. By comparing the deduced amino acid sequences of virulent and attenuated viruses, we identified sequence changes responsible for the adaptation and attenuation of the IBV-Ark DPI strain.
UR - http://www.scopus.com/inward/record.url?scp=62149104395&partnerID=8YFLogxK
U2 - 10.1007/s00705-009-0325-9
DO - 10.1007/s00705-009-0325-9
M3 - Article
C2 - 19219402
AN - SCOPUS:62149104395
SN - 0304-8608
VL - 154
SP - 495
EP - 499
JO - Archives of Virology
JF - Archives of Virology
IS - 3
ER -