Identification of recognition sites for Myc/Max/Mxd network proteins by a whole human chromosome 19 selection strategy

S. B. Akopov; I. P. Chernov; T. Wahlström; M. B. Kostina; G. Klein; M. Henriksson; L. G. Nikolaev

doi:10.1134/S0006297908110138

Identification of recognition sites for Myc/Max/Mxd network proteins by a whole human chromosome 19 selection strategy

S. B. Akopov, I. P. Chernov, T. Wahlström, M. B. Kostina, G. Klein, M. Henriksson, L. G. Nikolaev

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

In this study, we have identified 20 human sequences containing Myc network binding sites in a library from the whole human chromosome 19. We demonstrated binding of the Max protein to these sequences both in vitro and in vivo. The majority of the identified sequences contained one or several CACGTG or CATGTG E-boxes. Several of these sites were located within introns or in their vicinity and the corresponding genes were found to be up-or down-regulated in differentiating HL-60 cells. Our data show the proof of principle for using this strategy in identification of Max target genes, and this method can also be applied for other transcription factors.

Original language	English
Pages (from-to)	1260-1268
Number of pages	9
Journal	Biochemistry (Moscow)
Volume	73
Issue number	11
DOIs	https://doi.org/10.1134/S0006297908110138
State	Published - Nov 2008
Externally published	Yes

Keywords

Human chromosome 19
Max
Mxd
Myc
Target genes

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10.1134/S0006297908110138

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title = "Identification of recognition sites for Myc/Max/Mxd network proteins by a whole human chromosome 19 selection strategy",

abstract = "In this study, we have identified 20 human sequences containing Myc network binding sites in a library from the whole human chromosome 19. We demonstrated binding of the Max protein to these sequences both in vitro and in vivo. The majority of the identified sequences contained one or several CACGTG or CATGTG E-boxes. Several of these sites were located within introns or in their vicinity and the corresponding genes were found to be up-or down-regulated in differentiating HL-60 cells. Our data show the proof of principle for using this strategy in identification of Max target genes, and this method can also be applied for other transcription factors.",

keywords = "Human chromosome 19, Max, Mxd, Myc, Target genes",

author = "Akopov, \{S. B.\} and Chernov, \{I. P.\} and T. Wahlstr{\"o}m and Kostina, \{M. B.\} and G. Klein and M. Henriksson and Nikolaev, \{L. G.\}",

note = "Funding Information: This work was supported by grants from the Swedish Royal Academy of Science (M. H.), the Swedish Research Council (M. H.), and the Russian Foundation for Basic Research (01 04 48264) (L. N). S. Akopov, I. Chernov, and T. Wahlstr{\"o}m were all recipients of fellow ships from Cancer Research Institute and Concern Foundation, USA.",

year = "2008",

month = nov,

doi = "10.1134/S0006297908110138",

language = "English",

volume = "73",

pages = "1260--1268",

journal = "Biochemistry (Moscow)",

issn = "0006-2979",

publisher = "Pleiades Publishing",

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TY - JOUR

T1 - Identification of recognition sites for Myc/Max/Mxd network proteins by a whole human chromosome 19 selection strategy

AU - Akopov, S. B.

AU - Chernov, I. P.

AU - Wahlström, T.

AU - Kostina, M. B.

AU - Klein, G.

AU - Henriksson, M.

AU - Nikolaev, L. G.

N1 - Funding Information: This work was supported by grants from the Swedish Royal Academy of Science (M. H.), the Swedish Research Council (M. H.), and the Russian Foundation for Basic Research (01 04 48264) (L. N). S. Akopov, I. Chernov, and T. Wahlström were all recipients of fellow ships from Cancer Research Institute and Concern Foundation, USA.

PY - 2008/11

Y1 - 2008/11

N2 - In this study, we have identified 20 human sequences containing Myc network binding sites in a library from the whole human chromosome 19. We demonstrated binding of the Max protein to these sequences both in vitro and in vivo. The majority of the identified sequences contained one or several CACGTG or CATGTG E-boxes. Several of these sites were located within introns or in their vicinity and the corresponding genes were found to be up-or down-regulated in differentiating HL-60 cells. Our data show the proof of principle for using this strategy in identification of Max target genes, and this method can also be applied for other transcription factors.

AB - In this study, we have identified 20 human sequences containing Myc network binding sites in a library from the whole human chromosome 19. We demonstrated binding of the Max protein to these sequences both in vitro and in vivo. The majority of the identified sequences contained one or several CACGTG or CATGTG E-boxes. Several of these sites were located within introns or in their vicinity and the corresponding genes were found to be up-or down-regulated in differentiating HL-60 cells. Our data show the proof of principle for using this strategy in identification of Max target genes, and this method can also be applied for other transcription factors.

KW - Human chromosome 19

KW - Max

KW - Mxd

KW - Myc

KW - Target genes

UR - https://www.scopus.com/pages/publications/57249098373

U2 - 10.1134/S0006297908110138

DO - 10.1134/S0006297908110138

M3 - Article

C2 - 19120031

AN - SCOPUS:57249098373

SN - 0006-2979

VL - 73

SP - 1260

EP - 1268

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

IS - 11

ER -

Identification of recognition sites for Myc/Max/Mxd network proteins by a whole human chromosome 19 selection strategy

Abstract

Keywords

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