Identification of Nedd4 E3 ubiquitin ligase as a binding partner and regulator of MAK-V protein kinase

Svetlana V. Kalinichenko, Keiji Itoh, Elena V. Korobko, Sergei Y. Sokol, Vladimir L. Buchman, Igor V. Korobko

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

MAK-V/Hunk is a scantily characterized AMPK-like protein kinase. Recent findings identified MAK-V as a pro-survival and anti-apoptotic protein and revealed its role in embryonic development as well as in tumorigenesis and metastasis. However molecular mechanisms of MAK-V action and regulation of its activity remain largely unknown. We identified Nedd4 as an interaction partner for MAK-V protein kinase. However, this HECT-type E3 ubiquitin ligase is not involved in the control of MAK-V degradation by the ubiquitin-proteasome system that regulates MAK-V abundance in cells. However, Nedd4 in an ubiquitin ligase-independent manner rescued developmental defects in Xenopus embryos induced by MAK-V overexpression, suggesting physiological relevance of interaction between MAK-V and Nedd4. This identifies Nedd4 as the first known regulator of MAK-V function.

Original languageEnglish
Article numbere39505
JournalPLoS ONE
Volume7
Issue number6
DOIs
StatePublished - 20 Jun 2012

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