@article{5890a5c055b049219d48f399cd488773,
title = "Identification of microRNAs of the herpesvirus family",
abstract = "Epstein-Barr virus (EBV or HHV4), a member of the human herpesvirus (HHV) family, has recently been shown to encode microRNAs (miRNAs). In contrast to most eukaryotic miRNAs, these viral miRNAs do not have close homologs in other viral genomes or in the genome of the human host. To identify other miRNA genes in pathogenic viruses, we combined a new miRNA gene prediction method with small-RNA cloning from several virus-infected cell types. We cloned ten miRNAs in the Kaposi sarcoma-associated virus (KSHV or HHV8), nine miRNAs in the mouse gammaherpesvirus 68 (MHV68) and nine miRNAs in the human cytomegalovirus (HCMV or HHV5). These miRNA genes are expressed individually or in clusters from either polymerase (pol) II or pol III promoters, and share no substantial sequence homology with one another or with the known human miRNAs. Generally, we predicted miRNAs in several large DNA viruses, and we could neither predict nor experimentally identify miRNAs in the genomes of small RNA viruses or retroviruses.",
author = "S{\'e}bastien Pfeffer and Alain Sewer and Mariana Lagos-Quintana and Robert Sheridan and Chris Sander and Gr{\"a}sser, \{Friedrich A.\} and \{van Dyk\}, \{Linda F.\} and \{Kiong Ho\}, C. and Stewart Shuman and Minchen Chien and Russo, \{James J.\} and Jingyue Ju and Glenn Randall and Lindenbach, \{Brett D.\} and Rice, \{Charles M.\} and Viviana Simon and Ho, \{David D.\} and Mihaela Zavolan and Thomas Tuschl",
note = "Funding Information: We thank members of the Tuschl laboratory for helpful comments on the manuscript, and D. Marks, B. John and E. Clambey for discussions. M.Z. thanks E. van Nimwegen for useful suggestions. S.P. is supported by a grant from the Breast Cancer Alliance and by the Leukemia and Lymphoma Society (5554-05). A.S. is supported by the Swiss National Science Foundation grant 205321-105945 to M.Z.; B.D.L. and C.M.R. are supported by a grant from the US Public Health Service (CA85883). G.R. is supported by a grant from the American Cancer Society (PF-02-016-01-MBC). L.F.vD. is supported by the University of Colorado Health Science Center School of Medicine and Howard Hughes Medical Institutes Investigator Award and the US National Institutes of Health (CA103632). The research was also funded by a grant from the National Institutes of Health (P01 GM073047-01).",
year = "2005",
month = apr,
doi = "10.1038/nmeth746",
language = "English",
volume = "2",
pages = "269--276",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "4",
}