TY - JOUR
T1 - Identification of genes regulated by transcription factor KLF7 in differentiating olfactory sensory neurons
AU - Kajimura, Daisuke
AU - Dragomir, Cecilia
AU - Ramirez, Francesco
AU - Laub, Friedrich
N1 - Funding Information:
We thank Drs. F. Margolis, D. Anderson and R. Reed for providing critical reagents. We are also grateful for help from Dr. J. Zhang and her staff at the Cornell microarray facility. This work was supported by NIH grants AR38648, the New York Spinal Injury Research program, the St. Giles Foundation, and the James D. Farley family.
PY - 2007/2/15
Y1 - 2007/2/15
N2 - Gene targeting in mice has recently demonstrated that transcription factor KLF7 plays a critical role in neurite outgrowth and neuronal survival. Here we extended this genetic evidence by establishing the transcriptional profile of differentiating olfactory sensory neurons (OSNs) in Klf7-/- mice, and by identifying relevant genes that are directly regulated by KLF7. Functional clustering of DNA microarray data revealed that loss of KLF7 affects primarily the activity of genes involved in OSN differentiation, axonal growth, cytoskeletal dynamics, cell adhesion and synaptogenesis. Cell transfection experiments, on the other hand, demonstrated that the promoters of the genes encoding the OSN-specific OMP and the adhesion molecule L1 are both activated by KLF7 binding to CACCC motifs. Collectively, these results advance knowledge of transcriptional regulation of olfactory neurogenesis and KLF7 action.
AB - Gene targeting in mice has recently demonstrated that transcription factor KLF7 plays a critical role in neurite outgrowth and neuronal survival. Here we extended this genetic evidence by establishing the transcriptional profile of differentiating olfactory sensory neurons (OSNs) in Klf7-/- mice, and by identifying relevant genes that are directly regulated by KLF7. Functional clustering of DNA microarray data revealed that loss of KLF7 affects primarily the activity of genes involved in OSN differentiation, axonal growth, cytoskeletal dynamics, cell adhesion and synaptogenesis. Cell transfection experiments, on the other hand, demonstrated that the promoters of the genes encoding the OSN-specific OMP and the adhesion molecule L1 are both activated by KLF7 binding to CACCC motifs. Collectively, these results advance knowledge of transcriptional regulation of olfactory neurogenesis and KLF7 action.
KW - Axon growth
KW - Kruppel-like factor 7
KW - L1 adhesion protein
KW - Olfactory marker protein
KW - Olfactory sensory neuron differentiation
KW - Transcriptional regulation of neurogenesis
UR - http://www.scopus.com/inward/record.url?scp=33845797910&partnerID=8YFLogxK
U2 - 10.1016/j.gene.2006.09.027
DO - 10.1016/j.gene.2006.09.027
M3 - Article
C2 - 17123745
AN - SCOPUS:33845797910
SN - 0378-1119
VL - 388
SP - 34
EP - 42
JO - Gene
JF - Gene
IS - 1-2
ER -