Identification of genes regulated by transcription factor KLF7 in differentiating olfactory sensory neurons

Daisuke Kajimura, Cecilia Dragomir, Francesco Ramirez, Friedrich Laub

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Gene targeting in mice has recently demonstrated that transcription factor KLF7 plays a critical role in neurite outgrowth and neuronal survival. Here we extended this genetic evidence by establishing the transcriptional profile of differentiating olfactory sensory neurons (OSNs) in Klf7-/- mice, and by identifying relevant genes that are directly regulated by KLF7. Functional clustering of DNA microarray data revealed that loss of KLF7 affects primarily the activity of genes involved in OSN differentiation, axonal growth, cytoskeletal dynamics, cell adhesion and synaptogenesis. Cell transfection experiments, on the other hand, demonstrated that the promoters of the genes encoding the OSN-specific OMP and the adhesion molecule L1 are both activated by KLF7 binding to CACCC motifs. Collectively, these results advance knowledge of transcriptional regulation of olfactory neurogenesis and KLF7 action.

Original languageEnglish
Pages (from-to)34-42
Number of pages9
JournalGene
Volume388
Issue number1-2
DOIs
StatePublished - 15 Feb 2007
Externally publishedYes

Keywords

  • Axon growth
  • Kruppel-like factor 7
  • L1 adhesion protein
  • Olfactory marker protein
  • Olfactory sensory neuron differentiation
  • Transcriptional regulation of neurogenesis

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