@article{71706bdeb70f4f2b8545dc111164bd94,
title = "Identification of Ankrd2, a novel skeletal muscle gene coding for a stretch-responsive ankyrin-repeat protein",
abstract = "Mechanically induced hypertrophy of skeletal muscles involves shifts in gene expression leading to increases in the synthesis of specific proteins. Full characterization of the regulation of muscle hypertrophy is a prerequisite for the development of novel therapies aimed at treating muscle wasting (atrophy) in human aging and disease. Using suppression subtractive hybridization, cDNAs corresponding to mRNAs that increase in relative abundance in response to mechanical stretch of mouse skeletal muscles in vivo were identified. A novel 1100- bp transcript was detected exclusively in skeletal muscle. This exhibited a fourfold increase in expression after 7 days of stretch. The transcript had an open reading frame of 328 amino acids encoding an ATP/GTP binding domain, a nuclear localization signal, two PEST protein-destabilization motifs, and a 132-amino- acid ankyrin-repeat region. We have named this gene ankyrin- repeat domain 2 (stretch-responsive muscle) (Ankrd2). We hypothesize that Ankrd2 plays an important role in skeletal muscle hypertrophy. (C) 2000 Academic Press.",
author = "Kemp, {T. J.} and Sadusky, {T. J.} and F. Saltisi and N. Carey and J. Moss and Yang, {S. Y.} and Sassoon, {D. A.} and G. Goldspink and Coulton, {G. R.}",
note = "Funding Information: We thank Dr. L. Buluwela and Dr. S. Wynn for their advice and support and for supplying SON control primer and genomic DNA. We also thank Dr. Robert Brown UCL, London and Westminster University, London for their assistance with generation of muscle or-ganoids. Mr. Tim Kemp is supported by a BBSRC Prize Ph.D. Studentship, Ms. Teresa Sadusky is supported by a Muscular Dystrophy Campaign Prize Ph.D. Studentship, and Ms. Felicity Saltisi was supported by a Charing Cross and Westminster Medical School Alumni B.Sc. Studentship. Dr. Shi Yu Yang{\textquoteright}s salary was provided by a grant from the Wellcome Trust to work on the stretch response of muscle. Dr. David Sassoon is a recipient of a grant from the American Heart Association (Kenner Scholar) and the Muscular Dystrophy Association. Note. During this study another group submitted a cDNA sequence (Accession No. AJ011118, direct submission by Iev-olella et al., 1999) which differed by only a single basepair from Ankrd2. This may be a mouse strain polymorphism and does not alter the predicted amino acid sequence. They suggest expression within skeletal muscle and heart although no experimental data were provided.",
year = "2000",
month = jun,
day = "15",
doi = "10.1006/geno.2000.6213",
language = "English",
volume = "66",
pages = "229--241",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",
}