TY - JOUR
T1 - Identification of a purified complement fixing antigen as the Epstein Barr virus determined nuclear antigen (EBNA) by its binding to metaphase chromosomes
AU - Ohno, S.
AU - Luka, J.
AU - Lindahl, T.
AU - Klein, G.
PY - 1977
Y1 - 1977
N2 - A soluble complement-fixing antigen carried by Epstein-Barr virus (EBV)-transformed human cells has been previously extracted from cell nuclei and purified by DNA-cellulose chromatography. On addition of this antigen the methanol/acetic acid-fixed metaphase chromosomes, followed by exposure to human sera containing antibodies against the EBV-determined nuclear antigen (EBNA), brilliant positive staining was obtained by anti-complement immunofluorescence. There was no staining after exposure to EBV-negative sera. Moreover, a nuclear protein fraction, prepared from an EBV-negative cell line in an analogous fashion, failed to induce the staining reaction. These data identify the soluble purified antigen as the EBV-determined nuclear antigen. The purified antigen has a molecular weight of 174,000 ± 15,000, as determined by sucrose gradient centrifugation and gel filtration experiments. In neutral buffers containing 0.5-1.0 M NaCl, the antigen dissociates into a form of approximately one-half the original molecular weight with retained complement-fixing activity. This 'monomer' has a molecular weight of 98,000 ± 8,000.
AB - A soluble complement-fixing antigen carried by Epstein-Barr virus (EBV)-transformed human cells has been previously extracted from cell nuclei and purified by DNA-cellulose chromatography. On addition of this antigen the methanol/acetic acid-fixed metaphase chromosomes, followed by exposure to human sera containing antibodies against the EBV-determined nuclear antigen (EBNA), brilliant positive staining was obtained by anti-complement immunofluorescence. There was no staining after exposure to EBV-negative sera. Moreover, a nuclear protein fraction, prepared from an EBV-negative cell line in an analogous fashion, failed to induce the staining reaction. These data identify the soluble purified antigen as the EBV-determined nuclear antigen. The purified antigen has a molecular weight of 174,000 ± 15,000, as determined by sucrose gradient centrifugation and gel filtration experiments. In neutral buffers containing 0.5-1.0 M NaCl, the antigen dissociates into a form of approximately one-half the original molecular weight with retained complement-fixing activity. This 'monomer' has a molecular weight of 98,000 ± 8,000.
UR - http://www.scopus.com/inward/record.url?scp=0017595309&partnerID=8YFLogxK
U2 - 10.1073/pnas.74.4.1605
DO - 10.1073/pnas.74.4.1605
M3 - Article
C2 - 67603
AN - SCOPUS:0017595309
SN - 0027-8424
VL - 74
SP - 1605
EP - 1609
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 4
ER -