TY - JOUR
T1 - Identification of a novel group of evolutionarily conserved members within the rapidly diverging murine Cea family
AU - Zebhauser, Roland
AU - Kammerer, Robert
AU - Eisenried, Andreas
AU - McLellan, Andrew
AU - Moore, Tom
AU - Zimmermann, Wolfgang
N1 - Funding Information:
We thank Mirna Castro (Ludwig-Maximilians-University, Munich) for help with the sequencing, William Muller (McGill University, Montreal) for the gift of MMTV– c-neu- transgenic mice, and Nicole Beauchemin (McGill University, Montreal) for providing us with CT36 and CMT93 cells. This work was supported in part by the graduate program “Molecular Medicine” of the Medical Faculty of the Ludwig-Maximilians-University Munich (12/2003).
PY - 2005/11
Y1 - 2005/11
N2 - The carcinoembryonic antigen (CEA) family comprises a still actively evolving populous group of proteins that are involved in controlling tissue homeostasis, immune responses, and host/pathogen interactions. The genes identified to date in rodents and primates exhibit low sequence similarity and an extremely variable domain composition. Among the 22 murine Cea-related genes, only for Ceacam1 has an ortholog been assigned. To identify all CEA-related genes in mouse, rat, and human we undertook genome-wide analyses. Eight of 9 new expressible genes (Ceacam12-Ceacam20) could be located within the ∼6.5-Mb murine Cea locus. Five of the genes were rodent-specific (Ceacam12-Ceacam15 and Ceacam17). Surprisingly, for the remaining 4 (Ceacam16 and Ceacam18-Ceacam20) orthologs could be detected in all three genomes at syntenic locations. Gene-specific reverse transcription/PCR analyses of total RNA from 31 murine adult, placental, and embryonic tissues as well as tumors revealed very distinct expression patterns, suggesting diversified functions within the CEA family.
AB - The carcinoembryonic antigen (CEA) family comprises a still actively evolving populous group of proteins that are involved in controlling tissue homeostasis, immune responses, and host/pathogen interactions. The genes identified to date in rodents and primates exhibit low sequence similarity and an extremely variable domain composition. Among the 22 murine Cea-related genes, only for Ceacam1 has an ortholog been assigned. To identify all CEA-related genes in mouse, rat, and human we undertook genome-wide analyses. Eight of 9 new expressible genes (Ceacam12-Ceacam20) could be located within the ∼6.5-Mb murine Cea locus. Five of the genes were rodent-specific (Ceacam12-Ceacam15 and Ceacam17). Surprisingly, for the remaining 4 (Ceacam16 and Ceacam18-Ceacam20) orthologs could be detected in all three genomes at syntenic locations. Gene-specific reverse transcription/PCR analyses of total RNA from 31 murine adult, placental, and embryonic tissues as well as tumors revealed very distinct expression patterns, suggesting diversified functions within the CEA family.
KW - CEA family
KW - Carcinoembryonic antigen
KW - Cea gene locus
KW - Evolution
KW - Expression
KW - ITAM
KW - Pregnancy-specific glycoprotein
UR - http://www.scopus.com/inward/record.url?scp=26244455894&partnerID=8YFLogxK
U2 - 10.1016/j.ygeno.2005.07.008
DO - 10.1016/j.ygeno.2005.07.008
M3 - Article
C2 - 16139472
AN - SCOPUS:26244455894
SN - 0888-7543
VL - 86
SP - 566
EP - 580
JO - Genomics
JF - Genomics
IS - 5
ER -